HPLC法同时测定舒胆胶囊中芦荟大黄素和延胡索乙素的含量

HPLC simultaneous determination of aloe-emodin and tetrahydropalmatine in Shudan capsules

目的建立同时测定舒胆胶囊中芦荟大黄素和延胡索乙索含量的方法，为完善现有质量标准提供科学依据。方法采用HPLC法。用Diamonsil C18柱（200mm×4．6mm，5μm）分离，以甲醅pH6．8磷酸盐缓冲液（体积比70：30）进行等度洗脱，流速为1．0mL·min^-1，柱温为40℃，检测波长为280nm。结果芦荟大黄素质量浓度在0．1300～2．600mg·L^-1内、延胡索乙素质量浓度在4．352～87．04mg·L^-1内与峰面积呈良好的线性关系，相关系数分别为0．9997和0．9999；平均回收率分别为100．0％（RSD=0．81％）和100．1％（RSD=0．33％）。结论本方法简便、快捷，结果准确，重复性好。可应用于舒胆胶囊的质量控制。

Objective To establish an HPLC method for simultaneous determination of aloe-emodin and tetrahydropalmatine in Shudan （traditional Chinese medicines）capsules. Methods The chromatographic method was applied on a Diamonsil C18 column （200 mm × 4.6 mm, 5 μm） with methanol-pH 6.8 phosphate buffer （V： V = 70：30） as the mobile phase. The flow rate was 1.0 mL· min^-1 and column temperature was 40 ℃. The detection wavelength was set at 280 nm. Results The calibration curve was linear within the range of 0. 130 0 - 2. 600 mg· L^- 1 （ r = 0. 999 7） for aloe-emodin and 4. 352 - 87.04 mg· L^- 1 （ r = 0.999 9） for tetrahydropalmatine;the average recoveries were 100.0 % （RSD = 0.81% ） for aloe-emodin and 100.1% （RSD= 0.33 % ） for tetrahydropalmatine. Conclusions The method is simple, accurate and reproducible and can be used for the quality control of Shudan capsules.