缬沙坦对兔高脂血症肾脏的保护作用

Renoprotective Effects of Valsartan in Rats with Hyperlipemia

目的:观察兔高脂血症肾组织血管紧张素Ⅱ(AngⅡ)、核转录因子κB(NF-κB)、血管细胞黏附分子-1(VCAM-1)和碱性成纤维细胞生长因子(bFGF)的表达以及缬沙坦的干预作用。方法:24只健康雄性新西兰白兔随机分为3组,每组8只,正常对照组(A组)、高脂饮食组(B组)和治疗组(C组)。10周后,测定血清总胆固醇(TC)、三酰甘油(TG)和低密度脂蛋白(LDL)的浓度;HE染色观察肾组织病理改变,并用免疫组化方法测定AngⅡ、NF-κB、VCAM-1和bFGF的表达。结果:高脂血症时,肾组织同NF-κB、VCAM-1和bFGF表达增强;高脂饮食组相比,治疗组NF-κB、VCAM-1和bFGF在肾组织中的表达明显降低(P<0.05)。缬沙坦降低NF-κB、VCAM-1和bFGF在肾组织中的表达,减轻肾组织的炎症反应,抑制肾组织纤维化。结论:缬沙坦对兔高脂血症肾脏可能具有保护作用。

Objective： To observe the expression of angiotensin Ⅱ （Ang Ⅱ ）, nuclear factor-κB （NF-κB） ,vascular cell adhesion molecule 1 （VCAM-1） ,basic fibroblast growth factor （bFGF） and the intervention of valsartan. Methods： A total of 24 healthy male New Zealand white rabbits were randomly divided into three groups. They were a normal control group （group A ）, an atherogenic diet group（ group B） and a treatment group（ group C） （n = 8 in each group）. The concentrations of serum total cholesterol （TC）, triglyceride （TG） and low density lipoprotein （LDL） were detected by BackmaanLX20 automatic biochemical analyzer after ten weeks. Renal histopathological changes were observed by HE stain and the expression of Ang Ⅱ , NF-κB, VCAM-1 and bFGF was determined by immunohistochemistry. Results： There was a strong expression of renal tissues and NF-κB, VCAM-1 and bFGF in hyperlipedmia. Compared with the atherogenic diet group, the expression of NF-κB, VCAM-1 and bFGF in renal tissues of the treatment group decreased significantly （P 〈0.05）. Valsartan could reduce the expression of NF-κB, VCAM-1 and bFGF in renal tissues, relieve inflammatory response of renal tissues and inhibit renal fibrosis. Conclusion：Valsartan may have protective effects for renes of rats with hyperlipemia.