稻草秸秆同时糖化法制燃料酒精工艺条件

Fuel ethanol preparation from straw stalks using simultaneous saccharification and fermentation

以稻草秸秆为原料,对影响同时糖化法生产酒精的发酵温度、发酵时间、接种量、接种比例及纤维素酶用量等因素进行了优化研究。结果表明,稻草秸秆经过机械粉碎及稀硫酸预处理后,当发酵温度为38℃、接种量为10%、纤维素酶用量为40 IU/g、管囊酵母与酿酒酵母的接种比例为2∶1和发酵时间为72 h的条件下,酒精产率最高(为0.20 g/g稻草秸秆)。

The effects of temperature, time, inoculating quantity and dosage of cellulose enzyme on the preparation of fuel ethanol from straw stalks by means of the simultaneous saccharifieation and fermentation method （SSF） were studied. The results show that after pretreating straw stalk by mechanical comminution and sparse sulfuric acid, the optimal fermenting temperature is 38 ~C, the fermenting period is 72 h, the inoculating quantity is 10%, the dosage of cellulose enzyme is 40 IU/g, and the ratio of Pachysolen tannophilu to saccharomyces cerevisiae is 2： 1. The ethanol yield reaches as high as 0.20 g/g.