钴酞菁的微波合成及牛白蛋白测定

Synthesis of cobalt phthalocyanine under microave irradiation and determination of bovine albumin

以邻苯二腈、硫酸钴为原料，采用微波固相法合成了钴酞菁。将钴酞菁磺化所得的磺酸基钴酞菁溶液与牛白蛋白作用将导致共振瑞利散射显著增强。在460nm处存在一共振散射强峰，其强度增加与牛白蛋白的浓度呈线性关系，据此建立了一种用共振散射光谱测定牛白蛋白的方法。该方法的线性范围为0．1～6．0ug／mL，检测限为5．73ng／mL。

Cobalt phthalocyanine （CoPc）was prepared from phthalodinitrile and cobalt sulfate via microwave irradiation. The Resonance Rayleigh scattering（RRS）was enhnaced by the interaction of sulfonated CoPc and bovine albumin（BA）. An RRS peak formed at 460 nm with intensity proportional to the concentration of BA. A simple and rapid method of RRS for the determination of BA thus was established. The linear range is 0.1 ug/mL to-6.0 ug/mL. The detection limit for BA is 5.73 ng/mL.