乙型肝炎病毒基因型与原发性肝癌发生及家庭聚集性关系的研究

Relationship between hepatitis B virus genotype and occurrence as well as clustering of hepatocellular carcinoma in families

目的探讨乙型肝炎病毒(HBV DNA)基因型与广西原发性肝癌(HCC)发生及家庭聚集性的关系。方法采取配对方法选择广西肝癌高发区肝癌高发家庭成员340例及无癌家庭成员340例作为研究对象,应用荧光定量聚合酶链反应(FQ-PCR)结合荧光探针的体外DNA扩增检测技术测定HBV DNA,应用序列特异性寡核苷酸探针杂交技术(PCR-SSP)进行HBV DNA基因分型。结果①肝癌高发家庭成员HBV DNA的阳性率显著高于无癌家庭成员的阳性率(20.3%vs 11.2%,P<0.01);②随机抽取高发家庭成员组中HBV DNA阳性的42例进行基因型分析,B基因型占90.5%(38/42),A基因型占7.1%(3/42),C基因型占2.4%(1/42);而在无癌家庭成员组的35例中,B基因型占97.1%(34/35)例,A基因型占2.9%(1/35)例,C基因型未检出,两组比较差异无统计学意义(P>0.05),各组均未检测出D、E、F等基因型。结论HBV的感染及复制与广西肝癌的发生及家庭聚集性存在密切关系,但HBV DNA的基因型与广西肝癌的发生及家庭聚集性无明显关系。

Objective To explore the relationship between hepatitis B virus genotypes and the clustering of hepatocellular carcinoma（HCC） in families in Guangxi,China. Methods Pairing method was used to decide 340 cases from high-incidence HCC families and no HCC families in the prevalence area of liver disease in Guangxi. HBV DNA was detected by fluorescence quantitative polymerase chain reaction（FQ-PCR）, and polymerase chain reaction with sequence-specific primers（PCR-SSP） was employed to identify HBV DNA genotypes. Results The positive rate of HBV DNA in high-incidence HCC families was higher than that in no HCC families （20.3% vs 11.2%, P〈0.01）. Among 42 HBV DNA-positive subjects from high-incidence HCC families, the prevalence of genotype B,A, and C was 90.5 % （38/42）, 7.1% （3/42）, and 2.4 % （1/42）, respectively. The prevalence of genotype B, A, and C in 35 HBV DNA-positive subjects from no HCC families was 97.1% （34/35）, 2.9% （1/35） and 0% （0/35）, respectively, but no significant difference was shown between two groups （P〉0.05）, and genotype D, E, and F were not found in two groups. Conclusion Evident relationship exists between the infection and replication of HBV and the occurrence and clustering of HCC in families,but no significant relationship is found between the HBV genotypes and the occurrence clustering of HCC in families.