食管鳞状细胞癌组织中EphA2和EphrinA1的表达

Expression of EphA2 and EphrinA1 in esophageal squamous cell carcinoma

目的:观察食管鳞状细胞癌(食管鳞癌)组织中EphA2和EphrinA1蛋白及mRNA的表达水平,分析它们与食管癌患者预后的关系。方法:应用组织芯片技术和免疫组织化学方法检测173例食管鳞癌组织和19例正常食管黏膜组织中EphA2和EphrinA1蛋白表达水平;用激光捕获显微切割(LCM)技术和RT-PCR检测食管鳞癌组织中EphA2和EphrinA1 mRNA的水平。结果:食管鳞癌组织中EphA2阳性表达率80.9%、EphrinA1阳性表达率为84.4%,均明显高于正常食管黏膜(P<0.05);EphA2和EphrinA1蛋白表达同食管鳞癌患者的年龄、肿瘤部位、肿瘤大小、组织学分级无关;EphA2蛋白的表达水平与食管鳞癌患者的性别、淋巴结转移有关(P<0.05),EphrinA1蛋白的表达与食管鳞癌患者的临床分期有关(P<0.05)。生存分析显示:EphA2和EphrinA1蛋白的高表达与食管鳞癌患者的生存期有关。结论:EphA2和EphrinA1蛋白的高表达提示食管鳞癌患者预后不佳。

Aim： To observe the protein and mRNA expression of EphA2 and EphrinA1 in esophageal squamous cell carcinoma （ESCCs） , and analyze their relation with prognosis of ESCC. Methods ： Tissue mieroarray and immunohistochemistry techniques were used to detect the protein expression of EphA2 and EphrinA1 in both esophageal cancerous tissue and normal epithelial tissue; laser capture microdissection （LCM） and reverse transcription polymerase chain reaction （ RT- PCR） were used to study the mRNA expression of EphA2 and EphrinA1 in ESCC. Results： The protein and mRNA expres- sions of EphA2 and EphrinA1 in ESCC were markedly increased than those in the normal esophageal epithelia（ P 〈 0.05 ） ; EphA2 and EphrinA1 protein expressions were not associated with age, tumor location,tumor size or histological grade; but EphA2 protein expression was significantly associated with the gender （ P 〈 0.05 ） and lymph node metastasis（ P 〈 0.05 ） , and EphrinA1 protein expressions were significantly associated with clinieal stage（ P 〈 0.05）. Univariate analysis suggested that the protein overexpression of EphA2 and EphrinA1 was correlated with the survival time of patients with ESCC. Conclusion： The protein overexpression of EphA2 and EphrinA1 suggests poor prognosis for patients with ESCC.