戊四氮点燃大鼠海马Nogo-A及其受体NgR的表达

Expression of Nogo-A and its receptor NgR in hippocampus of rats kindled by pentyenetrazol

目的:探讨戊四氮点燃大鼠海马Nogo-A及其受体NgR的表达变化。方法:40只成年健康雄性大鼠随机分为实验组(腹腔注射戊四氮35mg/kg,1次/d)和正常对照组(腹腔注射生理盐水3.5mL/kg,1次/d),每组20只。实验第2周、4周,每组各取10只大鼠测定脑电图,然后采用免疫组织化学方法检测大鼠海马结构内Nogo-A及NgR的表达水平。结果:与对照组比较,第2周及4周时,实验组大鼠海马齿状回内分子层、门区及海马CA3区Nogo-A的表达降低(P<0.05),同时,实验组大鼠海马齿状回颗粒细胞层、门区及海马CA3区NgR的表达也降低(P<0.05)。结论:Nogo-A及NgR可能参与了癫后海马异常苔藓纤维出芽及突触重建过程。

Aim： To explore the expression of Nogo-A and its receptor NgR in hippocampus of rats kindled by pentyenetrazol. Methods：Forty healthy male rats were randomly divided into experimental group （ intraperitoneal injection of 35 mg/kg PTZ, daily）and control group （intraperitoneal injection of 3.5 mL/kg normal saline, daily） ,20 in each group. The expression of Nogo-A and its receptor NgR in the hippocampus of the rats were determined by immunohistochemistry. Results ： Compared with those of the control group, Nogo-A immunoreactivity was markedly decreased in the inner molecular layer in the dentate gyrus, hillus and the mossy fiber layer of the area CA3 in the hippocampus of the experimental rats （P 〈 0.05） at week 2 and week 4, and NgR immunoreactivity in the hippocampus of the experimental rats was simultaneously markedly decreased in the granular cell layer in the dentate gyrus, hillus and the pyramidal layer of the area CA3 in the hippocampus of the experimental rats （P 〈 0. 05 ）. Conclusion ： Nogo-A and NgR may be involved in the process of aberrant mossy fiber sprouting and synaptic plasticity in the hippocampus after seizure.