戊四氮点燃大鼠海马脑源性神经营养因子受体TrKB、P^(75NTR)表达的变化

Changes of TrKB,P^(75NTR) expression in hippocampus of rats kindled by pentylenetrazol

目的:探讨戊四氮点燃大鼠海马脑源性神经营养因子(BDNF)受体TrKB、P75NTR表达的变化。方法:40只成年健康雄性SD大鼠随机分为实验组和对照组,实验组采用戊四氮点燃法制备癫模型,对照组不做处理。第2周及第4周,2组各取10只大鼠,采用免疫组织化学方法观察海马结构内TrKB、P75NTR的表达水平。结果:第2周及第4周,对照组大鼠海马CA1、CA3区锥体细胞层及齿状回和门区可见TrKB阳性神经元和纤维;CA1、CA3区锥体细胞层、齿状回及门区可见大量P75NTR阳性神经元。与对照组比较,实验组海马相应部位TrKB的表达升高(P<0.05),P75NTR的表达降低(P<0.05)。结论:在癫形成过程中,BDNF的2类受体TrKB、P75NTR可能参与了癫后海马异常苔藓纤维出芽及突触重建过程;2类受体可能相互拮抗。

Aim ： TO explore the changes of TrKB, p^75NTR expression in hippocampus of rats kindled by pentyenetrazol. Methods：Forty healthy male rats were randomly divided into experimental group and control group. Rats in experimental group were kindled by pentyenetrazol. The expression of TrKB and p^75NTR in the hippocampus of the rats were determined by immunohistochemistry at Week 2 and Week 4. Results ： Compared with those of the control at Week 2 and Week 4, p^75NTR expression in the inner molecular layer of the dentate gyrus, hillus and the mossy fiber layer of the area CA1, CA3 in the hippocampus decreased（P 〈 0.05 ）, and TrKB expression in the hippocampus of the experimental rats increased（ P 〈 0.05 ）. Conclusion ： TrKB and p^75NTR may be involved in the process of aberrant mossy fiber sprouting and synaptic plastic- ity in the hippocampus after seizures, and the roles of the two receptors may be antagonistic.