托吡酯对戊四氮点燃大鼠海马白介素-1β表达的影响

Effect of topiramate on the expression of IL-1β in the hippocampus of rats kindled by pentyenetrazol

目的:探讨戊四氮(PTZ)点燃大鼠海马结构白细胞介素1β(IL-1β)表达的特征及托吡酯(TPM)对其表达的影响。方法:将30只成年健康雄性SD大鼠随机分为3组,每组10只。模型组大鼠胃灌入生理盐水(10mL/kg)1h后腹腔注射10 g/L PTZ生理盐水溶液(35mg/kg);TPM组大鼠按40mg/kg胃灌注10g/L TPM生理盐水溶液,1h后腹腔注射PTZ(剂量同模型组);对照组大鼠按10 mL/kg和3.5 mL/kg分别胃灌注和腹腔注射生理盐水。各组每d给药1次。当模型组大鼠性发作行为达到点燃标准时,对大鼠的行为进行观察和记录,采用免疫组织化学方法对各组大鼠海马结构IL-1β的表达进行检测。结果:实验第4周,模型组大鼠达到点燃标准;而TPM组大鼠最高发作级别为3级,均未达点燃标准;对照组大鼠行为正常。与对照组比较,模型组和TPM组大鼠海马结构IL-1β表达增强(P<0.05);TPM组大鼠海马结构IL-1β的表达较模型组降低(P<0.05)。结论:癫大鼠免疫系统处于活化状态,TPM能降低PTZ点燃大鼠海马结构IL-1β的表达而对癫具有一定的抑制作用。

Aim： To explore the expressional character of interleukin-1β （ IL-1β） and the effect of topiramate（TPM） on its expression in the hippocampus of rats kindled by pentyleneterazol（PTZ）. Methods： Thirty healthy male rats were randomly divided into three groups, ten in each group. The rats of group Ⅰ were given normal saline 10 mL/kg by gavage 1 h before intraperitoneal injection with 10 g/L PTZ at dose of 35 mg/kg daily. The rats of group Ⅱ were given 10 g/L TPM noral saline 40 mg/kg by garage 1 h before injection of 10 g/L PTZ at dose of 35 mg/kg daily. The rats of group Ⅲ （control group） were given the same amount of normal saline as other groups. When the spasm of rats in group Ⅰ reached kindling criterion, the behavior was observed and EEG was recorded, and immunohistochemistry was used to detect the IL-1β in the hippocampus of all rats. Results： The rats in group Ⅰ reached kindling criterion at Week 4, while the spasm scale of the rats in group Ⅱ was grade 3. Compared with that of group Ⅲ, IL-1β expression in the hippocampus of group Ⅰ and group Ⅱ significantly increased（P 〈 0.05）. Furthermore, the IL-1β expression of group Ⅰ was higher than that of group Ⅱ （P 〈 0.05 ）. Conclusion ： The immune system of the epileptic rat was greatly activated, TPM can decrease the expression of IL-1β in the hippocampus of epileptic rats, which might provide some protective action through immunosuppression.