缺血缺氧对星形胶质细胞合成和分泌BDNF的影响

Effects of ischemia and anoxia on astrocytic BDNF production in vitro

目的:观察缺血缺氧对星形胶质细胞合成和分泌脑源性神经营养因子(BDNF)的影响。方法:培养的星形胶质细胞分别在低氧去血清中培养1h、3h、6h、12h、1d、3d,采用逆转录PCR检测星形胶质细胞BDNF mRNA的表达,ELISA检测培养上清液中BDNF的浓度。结果:低氧去血清培养1h时星形胶质细胞BDNF mRNA表达达峰值,之后逐渐降低,培养12h后BDNF mRNA持续低于正常培养水平。在低氧去血清刺激下培养上清液中BDNF的浓度迅速增加,培养12h时达峰值,之后逐渐降低,保持低水平。结论:缺血缺氧促进星形胶质细胞活化,反应性增加BDNF的合成和分泌,这种变化与缺血后神经营养补给有关。

Aim ： To observe the effects of ischemia and anoxia on brain derived neurotrophic factor （BDNF） synthesis and secretion by astrocyte in vitro. Methods：BDNF mRNA in cultured astrocytes was tested by reverse transcriptase PCR,secreted BDNF in the supernatant was tested by ELISA, after 1 h, 3 h, 6 h, 12 h, 1 d, 3 d cultured in serum-free and low-oxygen medium, respectively. Results： The expression of BDNF mRNA reached peak at 1 h, then declined to lower level than normal after 12 h culture. BDNF in supernatant increased greatly in the first 12 h and reached peak at 12 h, then declined and maintained a stable level. Conclusion ： Increased production of astrocytic BDNF after ischemia and anoxia is an important supply of neurotrophic factors.