摘要
目的:观察缺血缺氧对星形胶质细胞合成和分泌脑源性神经营养因子(BDNF)的影响。方法:培养的星形胶质细胞分别在低氧去血清中培养1h、3h、6h、12h、1d、3d,采用逆转录PCR检测星形胶质细胞BDNF mRNA的表达,ELISA检测培养上清液中BDNF的浓度。结果:低氧去血清培养1h时星形胶质细胞BDNF mRNA表达达峰值,之后逐渐降低,培养12h后BDNF mRNA持续低于正常培养水平。在低氧去血清刺激下培养上清液中BDNF的浓度迅速增加,培养12h时达峰值,之后逐渐降低,保持低水平。结论:缺血缺氧促进星形胶质细胞活化,反应性增加BDNF的合成和分泌,这种变化与缺血后神经营养补给有关。
Aim : To observe the effects of ischemia and anoxia on brain derived neurotrophic factor (BDNF) synthesis and secretion by astrocyte in vitro. Methods:BDNF mRNA in cultured astrocytes was tested by reverse transcriptase PCR,secreted BDNF in the supernatant was tested by ELISA, after 1 h, 3 h, 6 h, 12 h, 1 d, 3 d cultured in serum-free and low-oxygen medium, respectively. Results: The expression of BDNF mRNA reached peak at 1 h, then declined to lower level than normal after 12 h culture. BDNF in supernatant increased greatly in the first 12 h and reached peak at 12 h, then declined and maintained a stable level. Conclusion : Increased production of astrocytic BDNF after ischemia and anoxia is an important supply of neurotrophic factors.
出处
《郑州大学学报(医学版)》
CAS
北大核心
2008年第3期480-482,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
国家自然科学基金资助项目C30400142
河南省科技厅重大攻关基金资助项目WKJ2007-2-031