来曲唑诱导的多囊卵巢综合征大鼠卵巢组织中角质细胞生长因子的表达

Expression of keratinocyte growth factor in ovary tissue of rats with letrozole-induced polycystic ovary syndrome

目的:探讨角质细胞生长因子(KGF)在多囊卵巢综合征(PCOS)发病机制中的作用。方法:40只雌性SD大鼠随机分为2组,PCOS组应用来曲唑诱导大鼠PCOS模型,对照组不作任何处理。阴道涂片观察大鼠动情周期变化;应用放免法测定大鼠血清促黄体生成素(LH)、促卵泡刺激素(FSH)、雌二醇(E2)、孕酮(P)和睾酮(T)水平;取卵巢组织行HE染色和免疫组织化学SABC染色分别观察组织学变化及卵巢组织中KGF的表达。结果:P-COS组大鼠失去规律性动情周期变化,血清T、LH浓度高于对照组(P<0.05),血清P浓度低于对照组(P<0.05);PCOS组KGF在窦前卵泡膜细胞的表达明显强于对照组(P<0.05),在囊状卵泡膜细胞的表达明显弱于对照组(P<0.05)。结论:来曲唑诱导的大鼠动物模型是理想的PCOS模型;KGF与PCOS的发病密切相关。

Aim： To investigate the role of keratinocyte growth factor（KGF） in the pathogenesy of polycystic ovary syndrome （PCOS）. Methods：Forty rats were divided into two groups randomly, PCOS group was induced PCOS by gavaging letrozole, and control group was not treated. The changes of estrus cycle were observed by means of vaginal smear every day, gonadal hormone concentrations in serum were determined by radioimmunoassay （RIA） , the histologic changes in ovaries were observed by HE staining and the expression of KGF in ovary tissue, by immunohistochemical methods. Results：The estrus cycles of PCOS group showed irregular change, which indicated that the rats had no ovulation. Serum testosterone and LH levels were elevated,and progestone levels were reduced （P 〈 0.05 ） ,which suggested that PCOS rat model was made successfully. In PCOS group, the expression of KGF in preantral follicles theca cells was obviously stronger and that in antral follicles theca cells was obviously weaker than those in the control group（ P 〈 0. 05 ）. Conclusion ： Letrozole-induced rat model is an ideal model of PCOS. KGF may be correlated with the pathogenesy of PCOS closely.