中草药雀儿舌头中总三萜酸含量的紫外分光光度法测定

Determination of the content of triterpenoidic acid in Leptopus Chinese (Bunge) Pojark

目的：测定雀儿舌头中总三萜酸的含量。方法：经过三萜酸中酚羟基与香草醛结构中的醛基进行缩合反应，生成红色的缩合物，采用紫外分光光度法测定缩合物的量，计算出总三萜酸的量。结果：该法的线性范围为40—180mg／L，r=0．9988，平均回收率为99．58％，RSD为1．03％（n=5）。雀儿舌头总三萜酸含量为1．0575％。结论：本法简便、快速，准确，重现性好，可用于雀儿舌头中总三萜酸含量的测定。

Aim：To determine the content of triterpenoidic acid in Leptopus Chinese （Bunge） Pojark. Methods：By using the uhraviolet spectrophotometrie method, the content of tritepenoidie acid in Leptopus Chinese （Bunge） Pojark was determinated. Results： The linear range was 40 - 180 mg/L, r = 0. 998 8. The averange recovery rate was 99.58% , and RSD was 1.03% （n =5 ）. The content of total triterpenoidic acid in Leptopus Chinese （Bunge） Pojark was 1. 057 5%. Conclusion： This method is simple,quick,accurate and reproducible and can be used for determining the content of tritepenoidic acid in Leptopus Chinese （Bunge） Pojark.