饱和D最优设计法在马铃薯微繁培养基筛选中的应用

Application of D-saturation optimum design in culture medium screening for micropropagation of virus-free tube potato

采用饱和D最优设计法，研究了5种植物生长调节剂与扦插个数的关系，通过记录青薯2号、6号两种脱毒试管苗生长状况，建立了不同植物生长调节剂、不同接种数对试管苗生长的函数模型。结果表明：试管苗最佳扦插个数：7—9个，2，4-D最佳浓度：1．32—2．514mg／L；6-BA最佳浓度：1．05—1．95mg／L；NAA最佳浓度：0．165—0．335mg／L；KT最佳浓度：0．35-0．65mg／L；CA最佳浓度：3．5～6．5mg／L。

The relationships between five plant growth regulars and inoculation number were explored by applying D-saturating optimum design. The function model was constructed by using growth indices of the plandets of two virus-free tube potato varieties Qingshu 2 and Qingshu 6. The results showed that the optimal inoculation number was within 7 ～ 9;the optimal concentration of 2,4-D was in the 1.32 mg/L and 2. 514 mg/L, the optina best concentration of 6-BA was between 1.05 rng/L and 1.95 mg/L, the best concentration of NAA was 0. 165 - 0. 335 mg/L , the best concentration of KT was 0. 35 -0.65 mg/L,and the best concentration of GA was 3.5 -6.5 mg/L