口腔鳞癌中PTEN基因表达与微血管密度的相关性研究

THE CORRELATION BETWEEN EXPRESSION OF PTEN AND MICROVESSEL DENSITY IN ORAL SQUAMOUS CELL CARCINOMA

目的:探讨口腔鳞癌组织中抑癌基因PTEN蛋白的表达与肿瘤微血管密度(MVD)之间的相关性。方法:采用免疫组化SP法检测10例正常口腔粘膜、10例口腔上皮单纯增生、15例口腔粘膜白斑及72例口腔鳞癌组织中PTEN基因和CD34的表达情况,分析与各临床病理因素和预后的关系。结果:PTEN基因在口腔鳞癌、口腔粘膜白斑、口腔上皮单纯增生和正常口腔粘膜组织中的阳性率分别为72.2%(52/72)、93.3%(14/15)、100%(10/10)和100%(10/10),口腔鳞癌组与其它各组间差异有统计学意义(P<0.05);PTEN的阳性表达率在不同性别、年龄、和TNM分期等临床病理参数间差异无统计学意义(P>0.05),但与淋巴结转移、组织分化程度等临床病理参数间有显著性差异(P<0.05)。PTEN蛋白表达与MVD呈显著负相关(P<0.05)。结论:PTEN基因表达的下调在OSCC的发生、发展中起着重要作用,PTEN可能通过某些途径抑制口腔鳞癌的血管生成。

Objective：To investigate the correlation between PTEN expression and microvessel density（MVD） in oral squamous cell carcinoma （OSCC）.Methods＇On 10 cases of normal oral mucosa ,10 cases of oral epithelial hyperplasia ,15 cases of oral leukoplakia and 72 cases of OSCC were immunohistochemical staining （S-P） performed for the expressions of PTEN and CD34,as well as MVD.Resuhs were compared with the clinical pathologic parameters and patients survival. Results： The positive rate of PTEN protein was 72.2% （52/72）, 93.3%（14/15）,100%（10/10） and 100%（10/10） in OSCC,oral leukoplakia ,oral epithelial hyperplasia and normal oral mucosa respectively,there was significant correlation among them （P 〈0.05 ）.PTEN expression was not obviously correlated with age, different gender, and TNM classification etc （P 〉0.05）,but significantly correlatedwith nodal metastases and degree of OSCC（P〈0.05）. FFEN expression significantly negatively correlated with MVD （r=-0.371 ,P〈0.001）. Conclusion：Down-regulation of PTEN protein expression may play an important role in the tumorigenesis and development of OSCC, PTEN gene negatively regulate the angiogenesis of OSCC.