重离子辐照对人肝癌HepG2细胞蛋白质组图谱的影响

Influence of Heavy Ion Irradiation on Proteome Profile of Human Hepatoma HepG2 Cells

分别制备经1 Gy C离子辐照和未经辐照人肝癌HepG2细胞的总蛋白样品,采用固相pH梯度双向凝胶电泳技术进行蛋白分离,用ImageMaster 2D双向电泳凝胶图像分析软件分析数字化图谱。结果显示,差异表达的蛋白质点为17个,其中1个仅在未经C离子辐照的HepG2细胞中表达,8个蛋白质点在辐照后的细胞中表达上调,8个蛋白质点在辐照后的细胞中表达下调。建立了重复性较好和分辨率较高的分离HepG2细胞总蛋白的双向电泳技术。实验结果表明,C离子辐照HepG2细胞后其蛋白质组发生了改变。

To gain a better understanding of the molecular basis of the biological effects induced by heavy ion irradiation, total proteins extracted from human hepatoma HepG2 cells that un-irradiated and irradiated with an intermediate-energy carbon ion beam at 1 Gy were separated by two-dimensional gel electrophoresis （2-DE） with immobilized pH gradients （IPGs）. The 2-DE profiles were analyzed with the ImageMaster 2D software. More than 500 proteins spots were reproducibly detected on each gel and 17 were differentially expressed for the un-irradiated and irradiated cells. 1 protein was expressed only in the un-irradiated cells.8 proteins were down-regulated and 8 proteins were up-regulated after the carbon ion irradiation. In this study, reproducible 2-DE profiles were established and some differential expressed proteins were detected.These basic data are very useful for the further study of heavy-ion biological effect.