经皮微波凝固治疗原发性肝癌局部巨噬细胞浸润变化的研究

The study of regional macrophage immune response on ultrasonically guided microwave coagulation therapy for primary hepatic carcinoma

目的研究超声引导下经皮微波凝固治疗原发性肝癌局部巨噬细胞浸润的变化。方法超声引导下经皮微波凝固治疗原发性肝癌患者89例，于治疗前后进行肝脏穿刺活检，经免疫组织化学染色后比较治疗前后局部巨噬细胞浸润的变化。电镜观察巨噬细胞的功能状态。结果经皮微波凝周治疗原发性肝癌后巨噬细胞局部浸润程度较治疗前明显增加，电镜观察该免疫细胞有伪足样突起，且胞质内可见较多内质网、线粒体及溶酶体。结论经皮微波凝固治疗原发性肝癌能有效地增强局部巨噬细胞的浸润程度和功能状态。

Objective To study the macrophage immune response after the pereutaneous microwave coagulation therapy （PMCT） under the ultrasound guide. Methods Eighty-nine cases of primary hepatic carcinoma patients were treated by PMCT. At the time of the fourth week after PMCT and before PMCT, the patients were given percutaneous biopsy under ultrasound guide. And the samples were stained by immunohistochemical methods. Then the infiltration of macrophage on local treatment areas were compared before and after the treatment. Moreover the function of immunocytes were observed by electronic microscope. Results The infiltration of macrophage increased significantly in post-PMCT as compared with that in pre-PMCT. And there were more protuberance from the macrophage and much more lysosome and endoplasmic reticulum and mitochondrium observed by electronic microscope. Conclusion The local macrophage cellular immunity can be more improved after the primary hepatic carcinoma treated by PMCT.