摘要
ISSR分析可对附子(Radix Aconiti Lateralis Preparata)的遗传多样性进行研究。为获得清晰、重复性高的ISSR扩增结果,对影响ISSR-PCR的多个因素,包括DNA模板浓度、Taq酶用量、引物和Mg2+浓度进行了比较、优化。研究表明,10-60ng模板DNA,1UTaq酶,0.2μmol/L引物,1.5mmol/LMgCl2,4种dNTPs各200μmol/L,10×PCR缓冲液构建的20μL的ISSR反应体系为最适体系。
The genetic diversity of Radix Aconiti Lateralis Preparata can be analyzed using inter-simple sequence repeats （ISSR） makers. Adjusting the influencing factors of ISSR with template DNA concentration, Taq polymerase content, primer concentration and Mg^2＋ concentration, the PCR amplification conditions were optimized. The optimal experiment conditions were as fellows： PCR reaction volume of 20L contained 10×PCR buffer, 10-60ng template DNA, 1U Taq polymerase, 0.2μmol/L primer and 1.5mmol/L MgCl2.
出处
《四川食品与发酵》
CAS
2008年第3期9-11,共3页
Sichuan Food and Fermentation
基金
四川省教育厅重点项目(2003A084)
关键词
附子
ISSR
优化
Radix Aconiti Lateralis Preparata
ISSR
optimize
