降解2，4-二氯酚的厌氧颗粒污泥-悬浮载体生物膜反应器中古细菌的种群结构

Community Structure of Archaea in Anaerobic Granular Sludge-suspended Carrier Biofilm Reactor for 2, 4-dichlorophenol Degradation

采用古细菌特异性引物ARC21F/ARC958R对污泥样品的总DNA进行聚合酶链式反应(PCR)、克隆、序列分析等,研究了降解2,4-二氯酚(2,4-dichloropheno,l2,4-DCP)的厌氧颗粒污泥-悬浮载体生物膜反应器(Anaerobic granu-lar sludge-suspended carrier biofilm reactor,ASBR)中古细菌的种群分布.结果表明,接种污泥和ASBR各层污泥中存在共有的古细菌:Methanothrix soehngenii和Uncultured archaeon等,M.soehngenii、uncultured archaeonTA05和unculturedarchaeon TA04在接种污泥和ASBR各层污泥中的分布为:接种污泥的丰度<上层的丰度<中层的丰度<下层的丰度.uncultured archaeon44A-1、uncultured archaeon39-2、uncultured archaeon46-1和uncultured archaeon69-1的分布为:接种污泥的丰度>上层的丰度>中层的丰度>下层的丰度.经2,4-DCP驯化后,ASBR上层悬浮填料区出现特有的古细菌unidentified crenarchaeote等,下层厌氧颗粒污泥区特有的古细菌为uncultured archaeon ZAR106等.接种污泥特有的6种古细菌Methanosaeta concilii等经2,4-DCP驯化后消亡,污泥中古细菌多样性减少,以下层颗粒污泥中古细菌种群丰度的变化最为明显.

Archaea community structure in anaerobic granular sludge-suspended cartier biofilm reactor （ASBR） for 2,4-di- chlorophenol degradation（2,4-DCP） was analyzed by 16S rDNA-based techniques. Total DNA were extracted directly from the top, middle and bottom layers of sludge in the ASBR and then amplified by polymerase chain reaction （PCR） with Archaea specific primer ARC21F/ARC958R. The positive PCR products were cloned and sequenced. The sequence analysis showed that there existed common Archaea in each layer of sludge in the ASBR and inoculated sludge, including Methanothrix soehngenii and uncultured archaeon. The abundance of M. soehngenii, uncultured archaeon TA05 and uncultured archaeon TA04 increased in the order： the inoculated sludge 〈 the top 〈 the middle 〈 the bottom in the ASBR. However, the abundance of uncultured archaeon 44A-1, uncultured archaeon 39-2, uncultured archaeon 46-1 and uncultured archaeon 69-1 declined in the order： the inoculated sludge 〉 the top 〉 the middle 〉 the bottom layer sludge in the ASBR. Some special Archaea were found, such as unidentified crenarchaeote in the top layer sludge and uncultured archaeon ZAR106 in the bottom layer sludge of the ASBR. Six Archaea originally existing in the inoculated sludge disappeared and the diversity of Archaea in the ASBR sludge decreased after 2,4-DCP-acclimation. The abundance of Archaea changed markedly in the bottom layer sludge of the ASBR.