大孔吸附树脂提取纯化甘草有效部位研究

Extraction and Purification of Effective Components of Glycyrrhiza with Macroporous Resin

目的:提取纯化甘草有效部位。方法:通过大孔吸附树脂,并以甘草酸和甘草苷的提取率为指标,优化提取纯化工艺。结果:DA201型大孔吸附树脂吸附性能优于D101型大孔吸附树脂,优化的工艺条件为上样7倍树脂柱体积的0.6倍原液浓度的样品液,流速2.5BV.h-1,吸附时间6h;12BV的70%乙醇洗脱。结论:甘草活性部位的得率为4.18%,其中含甘草酸78.40%,甘草苷17.51%。

Objective： To extract and purify the effective components of glycyrrhiza. Methods： To optimize the process of extraction and purification of glycyrrhiza, the contents of glycyrrhizic acid and liquiritin were the indexes with macroporous resin. Results ： The absorbing function of DA201 was better than D101 macroporous resin. The optimum technical operations were as follows： 0. 6 times concentration of original sample, 7 times the volume of the resin, the current velocity of absorbing was 2. 5BV·h^-1, and absorbed for 6 hours; eluted with 70% enthanol（12 times the volume of the resin） . Conclusion ： The yield of effective components of glycyrrhiza was 4. 18%, and the contents of glycyrrhizic acid and liquirtin were 78.40% and 17.51% separatedly.