摘要
目的观察蝙蝠葛酚性碱(PAMD)在体外的广谱抗肿瘤作用。方法采用MTT比色法测定不同质量浓度的蝙蝠葛酚性碱对体外处理白血病细胞株(K562)、人胰腺癌细胞株(BXPC-3)24 h的存活率,同时测定药物处理不同细胞株的细胞增殖抑制率和IC50及IC90。实验设立阴性空白对照组和氟尿嘧啶阳性对照组。结果不同质量浓度蝙蝠葛酚性碱对K562、BXPC-3细胞株的细胞增殖具有抑制作用,抑制作用均与质量浓度呈梯度关系。结论蝙蝠葛酚性碱对体外培养人肿瘤细胞株的增殖有明显的抑制和杀伤作用,揭示其具有细胞毒效应,蝙蝠葛酚性碱产生抗肿瘤生物学效应的机理有待进一步研究。
Objective To study broad-spectrum anti-tumor effect of phenolic alkaloids of Menispermum dauricum （PAMD） in vitro. Methods MTT colorimetric assay was employed to detect the survival rates of K562, BXPC-3 cell lines treated with PAMD of different concentration for 24 hours. Detected proliferation inhibition ratio, ICso and ICgo of different cell lines treated with PAMD of different concentration for 24 hours, established the negative blank control group and positive control group of 5-FU in the experiment. Results PAMD of different concentration could inhibit the proliferation of K562, BXPC-3 cell lines directly in a dosedependent. Conclusion PAMD could inhibit the proliferation of human cancer cells in vitro, this direct lethal and breakdown effect to cancer ceils shows its cytotoxic action. And the exact oncobiology effects of PAMD deserve further investigation.
出处
《甘肃中医学院学报》
2008年第3期1-4,共4页
Journal of Gansu College of Traditional Chinese Medicine
基金
黑龙江省科技攻关项目(D200675)
