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去污剂/聚合物双水相体系在膜蛋白分离中的应用 被引量：2

Application of Detergent/polymer Aqueous Two Phase Systems for Purification of Membrane Protein
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摘要 去污剂/聚合物双水相体系主要依靠胶束和聚合物分子间作用力来实现成相,具有对疏水膜蛋白增溶作用、成相物浓度低、萃取环境温和及操作简单等特点,可以克服传统膜蛋白提取工艺的一些缺陷;工艺开发过程中疏水膜蛋白以疏水作用富集于去污剂相,亲水蛋白以体积排阻效应富集于聚合物相,从而实现对膜蛋白的分离纯化。 Detergent/polymer aqueous two- phase systems （ATlaS）could improve the efficiency of membrane protein extraction, due to its advantages as follows ： （ 1 ） extraction of membrane proteins in the detergent - rich phase ; （2） low concentration of the phase - formed substances ; （ 3 ） the mild and convenient extraction conditions. Membrane proteins could be selectively partitioned into the micelle phase while hydrophilic proteins to the polymer phase to achieve its purification.
作者 刘娟 朱建航 范杰平
机构地区 南昌大学化学工程系
出处 《江西科学》 2008年第3期416-420,共5页 Jiangxi Science
关键词 去污剂 膜蛋白 胶束 双水相 Detergent, Membrane protein, Micelle, Aqueous two - phase systems
分类号 O6-332 [理学—化学] Q513 [生物学—生物化学]
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参考文献24

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共引文献73

同被引文献43

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江西科学
2008年 第3期

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