海南西岛九孔鲍养殖水体中细菌胞外产物的分析

Extra-cellular Factors from Bacteria Isolated from Abalone Farming Water

2004年11月,自海南西岛的鲍养殖水体中分离出90株菌株。TCBS和2116E培养基分别分离40株(弧菌)和50株(异养菌)。比较了不同分离方法所得到的2批菌株分泌胞外产物的能力。试验结果表明,鲍苗掉板死亡期间,养殖水体中TCBS培养基分离的菌株能分泌脂肪酶、淀粉酶、磷脂酶和明胶酶的比例总体上高于2116E培养基分离的菌株,2116E培养基分离的菌株中能分泌溶血素的比例要略高于TCBS培养基分离的菌株。总体上,后者具有较强分泌胞外产物的能力。因此,这部分具有较强分泌胞外产物能力的菌株应视为鲍苗的潜在致病菌。此外,在对鲍的细菌性病害研究中,除了具有较强分泌胞外产物的菌株外,养殖环境中的菌群结构也是不可忽视的因素。

Bacteria in abalone Haliotis diversicolor farming environment was found to be associated with abalone diseases. Ninety bacterial strains, in which 50 were isolated by 2116E plate （heterotrophic bacteria）, and 40 isolated by TCBS plate （Vibrio）, were isolated from an abalone farming water in Xidao, Hainan, China in November,2004. Their extra-cellular pathogetic factor-producing abilities were investigated. The results showed that there were more Vibrio （isolated by TCBS plate） excreting lipase, amylase, phospholipase and gelatinase than the heterotrophic bacteria isolated by 2116E plate. However, there were more bacteria （isolated by 2116E） excreting hemolysin than the bacteria isolated by TCBS plate. Overall, most vibrios had great abilities of producing extra-cellular products, and were considered as potential pathogens in the abalone farming environment. In addition, the bacterial community in farming environments should be take into consideration in dealing with abalone bacterial diseases.