哮喘患者诱导痰嗜酸细胞趋化因子的改变及其意义

The changes and significances of eotaxin levels in induced sputum from asthmatic patients

目的探讨嗜酸细胞趋化因子(嗜酸细胞趋化因子)在嗜酸细胞性气道炎症和气流阻塞发生中的作用。方法收集支气管哮喘(A组)急性发作期患者30例和健康对照者(B组)20例,分别给予肺功能检测和痰诱导检查。用酶联免疫吸附法(ELISA)测定诱导痰上清液中嗜酸细胞趋化因子浓度。结果A组诱导痰嗜酸细胞占白细胞百分比(EOS/Leu%)与B组比较,差异有显著性(P<0.05);A组嗜酸细胞趋化因子浓度与B组比较,差异有显著性(P<0.05)。A组EOS/Leu%、嗜酸细胞趋化因子浓度与一秒钟用力呼气容积占预计值百分比(FEV1%)均呈负相关;A组嗜酸细胞趋化因子浓度与EOS/Leu%呈正相关。结论嗜酸细胞趋化因子可能通过对嗜酸细胞(EOS)的选择性趋化作用使其释放一系列炎性介质参与了嗜酸细胞性气道炎症和气流阻塞的发生机制。

Objective Investigate the roles of eotaxin （chemotaetie factor of eosinophils） in pathogenesis of eosinophilie airway inflammation and airflow obstruction. Methods 30 patients in exacerbations with bronchial asthma （ A group） and 20 healthy volunteers as controls （ B group） have participated in lung function test and sputum induction. The concentration of eotaxin was measured with sandwich enzyme - linked immunosorbent assay （ELISA）. Results The differences of the eosinophil （EOS）/leukocyte （Leu） % in the two groups are significant （ P 〈 0, 05 ） ; The differences of the eotaxin in the two groups are also significant （ P 〈0.05 ） ; The EOS/Leu% and the level of eotaxin are significantly inversely correlated with the percentage of the first second forced expiratory volume / predicted value （ FEVI% ） （ P 〈0. 001 ）. The level of eotaxin in induced sputum are significantly positively correlated with the EOS/Leu% in A group （ P 〈 0. 001 ）. Conclusion Eotaxin possiblely particulates in eosinophilic airway inflammation and airflow obstruction in asthmatic patients by selective chemotactic activity on eosinophil, and eosinophil release a series of inflammation medium that induce the exacerbation of asthma.