高效液相色谱法测定抗骨增生丸中淫羊藿苷的含量

Determination of icariin in kangguzengsheng pills by HPLC

目的建立抗骨增生丸中淫羊藿苷含量的高效液相色谱法测定方法。方法样品经处理后,由Diamonsil C18（4.6mm×200mm,5μm）色谱柱分离,流动相：乙腈-水（26∶74）,流速：1.0mL.min^-1,检测波长：270nm。结果方法分离良好,样品中杂质不干扰淫羊藿苷的分离测定,淫羊藿苷浓度在0.0404-0.504μg内与峰面积线性关系好,r=0.9999,平均回收率为98.4%,RSD为0.7%（n=6）。结论本法测定简便,结果准确,重复性和分离度好,可用于抗骨增生丸的质量控制。

Objective To develop an HPLC method for the determination of icariin in Kangguzengsheng pills.Methods A C18 column（4.6 mm×200 mm,5 μm）,and a mobile phase composed of acetonitrile-water（26∶74）were adopted.The flow rate was 1.0 mL·min^-1 and wave length was 270 nm.Results The linear range was 0.040 4～0.504 μg,r=0.999 98.The recovery of paeoniflorin was 98.4% with RSD=0.7%（n=6）.Conclusion The determination method is simple and accurate,and can be used for the quality control of Kangguzengsheng pills.