两个大珠母贝群体遗传多样性的ISSR分析

ISSR analysis of genetic diversity in two populations of pearl oyster Pinctada maxima

用ISSR标记技术对我国两个大珠母贝Pinctada maxima群体的遗传多样性进行分析。7条ISSR引物在两个群体中共得到91个清晰的扩增位点。总多态位点百分率为100%,H=0.2832,I=0.4372,总的遗传变异中有21.54%的变异存在于两个群体之间,而78.46%的遗传变异是发生在群体内。海南群体的遗传多样性水平(PPB=91.21%,I=0.4144±0.2364,H=0.2715±0.1790)高于广西群体(PPB=82.42%,I=0.3621±0.2534,H=0.2356±0.1837),广西群体的单态位点数(15个)明显高于海南群体(8个)。NJ聚类分析表明两群体各自聚类成一簇。研究结果表明我国大珠母贝的遗传多样性水平较高。

Genetic diversity within and between two geographical populations of Pinctada maxima from Hainan and Guangxi provinces in China was determined using inter simple sequence repeats （ISSR）. Seven primers produced 91 bands across a total of 80 individuals. The genetic diversity was high at species level （PPB = 100%, H = 0. 283 2, and I = 0. 437 2）, 21.54% of the total genetic variation resided between the two populations and 78.46% within the populations. Higher genetic diversity level was observed in the Hainan population （PPB = 91.21%, H = 0. 271 5, and I = 0. 414 4） compared with the Guangxi population （PPB = 82.42%, H = 0. 235 6, and I = 0. 362 1）; however, the number of monomorphic sites of the Guangxi population （15） was greater than that of the Hainan population （8）. NJ Cluster analysis divided the populations into two main groups. The results of this study suggested that there is a higher genetic diversity level in P. maxima populations in China and greater genetic variation between the Guangxi and Hainan populations.