摘要
洛美沙星(LFLX)在紫外光照射下易发生光化学降解反应,光解产物(ILFLX)与Tb3+形成的配合物对Ce(Ⅳ)-SO32-化学发光体系有较强的增敏作用,与光解前体系的化学发光强度相比,增大近5倍。据此,建立了光化学-能量转移化学发光法测定LFLX的新方法。该方法的线性范围为8.0×10-10~2.0×10-5mol/L;检出限为2.5×10-10mol/L;相对标准偏差(n=11,Cs=5.0×10-8mol/L)为2.8%。利用该法测定了生物样品中LFLX的含量,结果满意。并在实验的基础上,对其光化学增敏反应机理进行了合理的推测。
A photodegradation reaction took place when the lomefloxacin (LFLX) was irradiated by ultraviolet radiation, and the photoproduct can form a complex with Tb^3 +. The complex can sensitize the chemilumines-cence(CL) reaction of Ce(IV)-SO3^2- , its emission intensity increased about five times than the non-irradiated system. Based on the CL reaction, a new photochemical energy transfer CL method for the determination of LFLX was developed. The relative CL intensity was linear with the concentration of LFLX in the range of 8.0×10^-10 -2.0×10^-5 mol/L. The detection limit is 2.5×10^-11 mol/L. The relative standard deviation for the determination of 5.0×10^-8 mol/L LFLX was 2.8% ( n = 11 ). The method was successfully applied to the determination of LFLX in biological fluid. The mechanism of this system is proposed according to the results of experiment.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2008年第6期823-826,共4页
Chinese Journal of Analytical Chemistry
基金
江苏省“青蓝工程”资助课题
关键词
光化学降解
洛美沙星
能量转移化学发光
Photodegradation, lomefloxacin, energy transfer chemiluminescence