期刊文献+

能量转移化学发光法测定洛美沙星 被引量:3

Energy Transfer Chemiluminescence Determination of Lomefloxacin
下载PDF
导出
摘要 洛美沙星(LFLX)在紫外光照射下易发生光化学降解反应,光解产物(ILFLX)与Tb3+形成的配合物对Ce(Ⅳ)-SO32-化学发光体系有较强的增敏作用,与光解前体系的化学发光强度相比,增大近5倍。据此,建立了光化学-能量转移化学发光法测定LFLX的新方法。该方法的线性范围为8.0×10-10~2.0×10-5mol/L;检出限为2.5×10-10mol/L;相对标准偏差(n=11,Cs=5.0×10-8mol/L)为2.8%。利用该法测定了生物样品中LFLX的含量,结果满意。并在实验的基础上,对其光化学增敏反应机理进行了合理的推测。 A photodegradation reaction took place when the lomefloxacin (LFLX) was irradiated by ultraviolet radiation, and the photoproduct can form a complex with Tb^3 +. The complex can sensitize the chemilumines-cence(CL) reaction of Ce(IV)-SO3^2- , its emission intensity increased about five times than the non-irradiated system. Based on the CL reaction, a new photochemical energy transfer CL method for the determination of LFLX was developed. The relative CL intensity was linear with the concentration of LFLX in the range of 8.0×10^-10 -2.0×10^-5 mol/L. The detection limit is 2.5×10^-11 mol/L. The relative standard deviation for the determination of 5.0×10^-8 mol/L LFLX was 2.8% ( n = 11 ). The method was successfully applied to the determination of LFLX in biological fluid. The mechanism of this system is proposed according to the results of experiment.
出处 《分析化学》 SCIE EI CAS CSCD 北大核心 2008年第6期823-826,共4页 Chinese Journal of Analytical Chemistry
基金 江苏省“青蓝工程”资助课题
关键词 光化学降解 洛美沙星 能量转移化学发光 Photodegradation, lomefloxacin, energy transfer chemiluminescence
  • 相关文献

参考文献12

二级参考文献23

  • 1李惠明,宣坚钢.盐酸洛美沙星片的紫外分光光度测定[J].中国医药工业杂志,1995,26(12):546-548. 被引量:10
  • 2[1]Baswaraj J, Ravisankar S, Vasudevan M, Suresh B. East Pharm., 1996, 39(462):127~128
  • 3[2]Reddy M N, Reddy M V V N, Sridevi D, Sushruta K, Sankar D G. J. Inst. Chem., 2000, 72 (1):32~35
  • 4[3]Zhang, Tieli, Zhao, Huichun, Jinpei. Talanta, 1999, 49 (1):77~82
  • 5[4]Yorke J C, Froc P. J. Chromatogr.A, 2000, 882(1-2):63~77
  • 6聂青松,北京医科大学学报,1979年,1卷,64页
  • 7张致平,中国抗生素杂志,1995年,20卷,79页
  • 8张致平,引进国外医药技术与设备,1995年,4卷,216页
  • 9中国抗生素杂志,1992年,17卷,87页
  • 10Chu D P W,31st Antimicrobial Agents and Chemotherapy,1991年,1440页

共引文献155

同被引文献60

引证文献3

二级引证文献7

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部