万古霉素及其苯异氰酸酯衍生物手性固定相的制备与应用

Preparation and Application of Vancomycin and Its Phenyl Isocyanate Derivative Chiral Stationary Phase

采用"一锅法",以1,6-二异氰酸正己酯作间隔臂,制备了万古霉素及苯异氰酸酯衍生化的万古霉素手性固定相。对拉米夫定、拉米夫定的L-薄荷醇酯、酞胺哌啶酮和盐酸氟西汀进行了手性分离研究,在极性有机相模式下,研究了流动相甲醇中冰醋酸-三乙胺浓度和比例对手性分离的影响,观察到两种手性固定相具有不同的手性识别能力。在万古霉素手性固定相上4种溶质都获得了基线分离;在苯异氰酸酯衍生化的手性固定相上除盐酸氟西汀外也均获得基线分离。

Using 1,6-diisocyanatohexane as spacer, the chiral stationary phases（CSPs） of vancomycin and its phenyl isocyanate derivative were prepared by “one-pot” synthetic strategies. The CSPs were used to separate the enantiomers of larnivudine, its ester of L-menthol, thalidomide and fluoxetine hydrochloride, under polar organic phase, the influences of mobile phase composition, such as the concentration and ratio of glacial acetic acid and triethylamine in methanol, on the enantioseparation were investigated, it was found that the two chiral stationary phases had different chrial recognition ability, the four solutes was got baseline separation on vancomycin chiral stationary phase, and also got baseline separation except fluoxetine hydrochloride on vancomycin phenyl isocyanate derivative chiral stationary phase.