吸入氡致大鼠肺蛋白质组表达变化的分析

Proteomic analysis of lung protein expression in rats inhaled radon

目的从蛋白质组学角度分析氡染毒后大鼠肺组织蛋白质表达谱的变化。方法雄性Wiser大鼠氡吸入染毒累积剂量分别达100、200、400工作水平月（WLM）后，取大鼠肺组织，裂解提取其总蛋白；双向电泳（2-DE）分离总蛋白，Image Master 2D Platinum软件分析差异表达蛋白，切取差异蛋白点，胶内酶解并进行质谱（MALDI-TOF-MS）鉴定。结果正常对照组和氡染毒组大鼠肺组织的2-DE图谱有明显差异，差异表达点中与氡染毒呈剂量变化关系的点有14个表达上调，9个表达下调，质谱鉴定出其中的15个差异蛋白点。结论氡染毒组大鼠肺组织的蛋白表达谱发生了一定的差异性，氡吸入致靶器官肺损伤可能与多种蛋白相关。

Objective To detect the changes in protein profile of rats＇ lung inhaled radon using two- dimensional electrophoresis （2-DE）and MS. Methods Male Wistar rats were exposed to radon with the cumulative dose up to 100, 200 and 400 working level months （WLM）. The total proteins of rats＇ lung were extracted and isolated by 2-DE. The different protein expressions were analyzed with ImageMaster 2D Platinum software and obviously altered proteins were digested by trypsin and identified by MALDI-TOF-MS. Results Comparison of the 2-DE images between the control and radon-exposed groups indicated 14 up-regulated and 9 down-regulated protein spots, 15 of which were identified by MALDI-TOF or MALDI-TOF/TOF MS. Conclusions The 2-DE image of radon groups＇ lung has changed, and the lung damage effect of radon is related to some proteins.