神经生长因子和氨基胍对糖尿病大鼠视觉诱发电位的影响

Influence of nerve growth factor and aminogunidine on visual evoked potential in diabetic rats

目的:观测糖尿病大鼠视觉诱发电位(VEP)的改变以及神经生长因子(NGF)和氨基胍(AG)对其的影响。方法:用链脲佐菌素(STZ)制作糖尿病大鼠动物模型,分为正常对照组(CON组)、糖尿病对照组(DM组)、糖尿病NGF治疗组(D+N组)、糖尿病AG治疗组(D+A组)。分别于病程3、6、9、12个月进行各组大鼠的VEP测定。结果:与CON组相比,其他各组大鼠在各实验时点的VEP潜伏期延长、波幅下降(P<0.01);6个月及9个月时,D+N组及D+A组的潜伏期均较DM组缩短,且差异显著(P<0.05).而波幅仅见6个月时部分回升;12个月时,除D+A组波幅较DM组略有回升外,其余改变与9个月时类似。结论:NGF和AG能够改善DM导致的大鼠VEP潜伏期延长及波幅下降。

Objective：To observe the changes of visual evoked potential （VEP） in diabetic rats and the influence of nerve growth factor （NGF） and aminoguanidine （AG） on VEP. Methods： Diabetes was induced in adult male Wistar rats with streptozotocin （STZ）. Rats were divided into normal control group （CON）,diabetes model group （DM）, NGF-treated group （D＋N） and AG-treated group（D＋ A）. VEP was measured during the 3rd month, 6^th month, 9^th month,and 12^th month. Results： Compared with the CON group,all rest groups had longer latencies and lower amplitudes （P〈0. 01）. During the 6^th and 9^th months, the latencies in group D＋N and group D＋A were significantly decreased （P〈0.05） compared with DM group, and the amplitude was partially recoved during the 6^th month. The changes during the 12^th month were similar to those of the 9^th month, except that the amplitude in D＋A group was slightly higher than that in the DM group. Conclusion： NGF and AG can improve DM-induced elongation of VEP latency and decrease of VEP amplitude.