摘要
目的:定量检测转化生长因子-(受体Ⅰ(transforming growth factor-(typeⅠreceptor,TβRⅠ)和受体Ⅱ(TβRⅡ)基因在大鼠视网膜中的表达水平,探讨TGF-(不同受体在视网膜中表达的差异及其意义。方法:分离取出大鼠视网膜,抽提RNA并逆转录,实时荧光定量PCR技术分析视网膜中TβRⅠ和TβRⅡ的mRNA含量。结果:TβRⅠ相对于18S的mRNA含量是0.00034±0·00013,TβRⅡ相对于18S的mRNA含量是0.0001±0·00005,差异有统计学意义(P<0.01)。在大鼠视网膜中以TβRⅠ表达为主,TβRⅠ和TβRⅡ比值的平均值为3·9±1.7。结论:实时荧光定量PCR技术能够针对性地精确分析极少量组织细胞的基因表达,TβRⅠ的mRNA在视网膜中的表达明显高于TβRⅡ,提示这可能是与TβRⅠ及TβRⅡ本身结构特点和在TGF-β信号转导过程中的不同作用有关。当TβRⅠ/TβRⅡ比例改变时,可影响细胞对TGF-β的应答反应,可能是增殖性视网膜病变的发生机制之一。
AIM- To quantitatively investigate transforming growth factor-β type Ⅰ receptor （TβR Ⅰ） and transforming growth factor-β type Ⅱ receptor （TβR Ⅱ） gene expressions in rat retina.METHODS： Sprague-Dawley rats were chosen in this research. Gene expression was detected quantitatively by reverse transcription polymerase chain reaction （RT-PCR） analysis. RESULTS： The expression level of TβR Ⅰ and TβR Ⅱ were 0.00034 ± 0. 00013 and 0.0001 ±0.00005, respectively. The expression level of TβR Ⅰ was obviously higher than that of TβR Ⅱ in the rat retina with statistical significance （P〈 0.01）. The ratio of TβR Ⅰ/TβR Ⅱ was 3.9 ±1.7. CONCLUSION： Real time quantitative RT-PCR is an effective method to detect differential expression genes in retina. The change of TβR Ⅰ/TβR Ⅱ expression may play an important role in the pathogenesis of retinopathy, which could be further investigated in its significance in the development of proliferation retinopathy.
出处
《国际眼科杂志》
CAS
2008年第6期1073-1075,共3页
International Eye Science
基金
中国国家自然科学基金资助项目(No.30271391)
中国上海市卫生局科研项目(No.034124)
中国上海市卫生系统百名跨世纪优秀学科带头人培养计划(百人计划)基金资助项目(No.057)~~
关键词
转化生长因子-Β受体
实时荧光定量PCR
基因
表达
视网膜
TGF-β receptor
quantitative reverse transcription polymerase chain reaction （QRT-PCR）
gene expression
retina
