哈蟆油胶囊对衰老模型小鼠生殖器官的保护作用

Protective effect of Oviductss Ranae capsules on the reproductive organs of aged mice

目的以D-半乳糖所致雌性衰老模型小鼠为实验对象,观察哈蟆油胶囊(OR)对其生殖器官的保护作用。方法采用雌性昆明小鼠48只,随机分为OR高、低剂量组和阳性药对照组、模型对照组,每组12只,D-半乳糖颈背部皮下注射6周。另设一组12只,同样部位每日注射生理盐水,作为正常对照组。第3周开始灌胃给药,模型对照组和正常对照组灌胃植物油。最后2周逐只进行阴道脱落细胞涂片,使用瑞氏-姬姆萨染液染色后观察其阴道角化细胞变化,计数动情期天数。给药结束后收集标本,采用放免法测定血清雌二醇水平,测定卵巢和子宫指数,观察卵巢和子宫病理变化,检测卵巢子宫中SOD以及MDA的水平。结果OR明显提高老年小鼠体内雌激素水平,延长小鼠动情期;增加其卵巢和子宫指数,改善卵巢和子宫的病理改变,降低卵巢子宫中MDA含量,提高其SOD活力。结论OR能够延缓雌性小鼠生殖衰老,增强生殖器官抗氧化作用,从而改善其萎缩和衰老程度。

Objective To observe the protective effect of Oviductus Ranae （OR） capsules on the reproductive organs in an aged mouse model established by D-galactose injection. Methods Forty-eight female Kunming mice were randomly divided into 4 equal groups, namely the high- and low-dose OR groups, diethylstilbestrol （DT） group, and model group. The mice received subcutaneous injection of D-galactose for 6 weeks to establish aging models. Another 12 mice were ＇.rejected daily with normal saline （NS） to serve as the normal control group. From the third week of the experiment, the mice were given oral OR at low or high doses （in the OR groups） or vegetable oil （in the model or control groups） till the sixth week. In the last two weeks, the vaginal smears were obtained from the mice for evaluating the changes of the vaginal keratinocytes and counting the days of estrus. After completion of drug administration, all the mice were sacrificed and the serum content of estradiol （E2） was detected by radioimmunoassay, with the ovarian and uterine indices determined. The ovarian and uterine pathologies were observed using HE staining, and SOD and MDA activities in the ovary and uterus were also assessed. Results OR obviously increased E2 level and the ovarian and uterine indices in the aged mice, also alleviating the pathological change of the ovary and uterus. OR substantially depressed MDA content and enhanced SOD activity in the ovary and uterus. Conclusion OR has definite antioxidative effects and ameliorates the degenerative changes of the reproductive organs in mouse models of aging.