湖南汉族白血病与HLA-A、B、DRB1基因的相关性研究

Association of HLA-A,B and DRB1 alleles with leukemia in Han population in Hunan Province

目的探讨湖南汉族白血病与HLA-A、B、DRB1基因的相关性。方法应用PCR/SSP方法对湖南汉族等待骨髓移植的105例慢性粒细胞性白血病(CML)、25例急性淋巴细胞性白血病(ALL)和48例急性非淋巴细胞性白血病(ANLL)患者进行HLA-A、B、DRB1基因分型,并以3664例湖南汉族无关健康个体的造血干细胞HLA分型资料作为正常对照。结果CML患者B58、DR12、DR14基因表型频率明显高于对照组,RR分别为6.1287、1.6519、1.6479;ALL患者B58基因表型频率明显高于对照组,RR为7.4055;ANLL患者B58、DR8基因表型频率明显高于对照组,RR分别为13.9789、2.2839;ANLL患者A24基因表型频率明显低于对照组,RR为0.4012。结论HLA-B58、DR12、DR14可能是CML的易感基因;HLA-B58可能是ALL的易感基因;HLA-B58、DR8可能是ANLL的易感基因,而HLA-A24可能是ANLL的保护基因。

Objective To investigate the association of HLA-A, B, and DRB 1 alleles with leukemia in the Han population in Huan Province. Methods HLA-A, B, and DRB1 alleles were genotyped in 105 patients with chronic myelocytic leukemia, 25 with acute lymphocytic leukaemia, and 48 with acute nonlymphocytic leukemia using polymerase chain reaction with sequence-specific primer （PCR-SSP）. The hemopoietic stem cells from 3664 unrelated normal individuals of Han nationality in Hunan were used as the control group. Results The phenotypic frequencies of HLA-B58, DRI2, and DRI4 were significantly higher in patients with chronic myelocytic leukemia than in the control group, with relative risk of 6.1287, 1.6519, and 1.6479, respectively. In patients with acute lymphocytic leukaemia, the phenotypic frequency of HLA-B58 was significantly higher than that in the control group, with the relative risk of 7.4055. In patients with acute nonlymphocytic leukemia, the fi＇equencies of HLA-B58 and DR8 phenotypes were significantly higher but HLA-A24 frequency was significantly lower than those of the control group, with the relative risk of 13.9789, 2.2839, and 0.4012, respectively. Conclusions HLA-B58, DRI2, DRI4 alleles appear to contribute to the genetic susceptibility of patients with chronic myelocytic leukemia. HLA-B58 allele can be associated with the genetic susceptibility for patients with acute lymphocytic leukaemia. In patients with acute nonlymphocytic leukemia, HLA-B58 and DR8 are probably the susceptible alleles whereas HLA-A24 allele may play a protective role.