PPARγ对实验性胰腺炎大鼠多器官功能的作用

Effects of peroxisome proliferators-activated receptor-γ on the function of the vital organs in rats with pancreatitis

目的研究PPARγ对实验性胰腺炎大鼠多器官功能的作用。方法SD雄性大鼠随机分为4组,每组10只。①正常对照组;②胰腺炎模型组采用胆胰管注射质量分数4%的牛磺胆酸钠,剂量为1.0ml/kg,制成胰腺炎模型;③PPARγ配体罗格列酮处理组于造模成功后,静脉注射罗格列酮0.3mg/kg;④PPARγ拮抗剂GW9662处理组于造模成功后,静脉注射GW96620.3mg/kg,10min后再次静脉注射罗格列酮0.3mg/kg。各组于实验6h后采集血样并处死大鼠,检测各组胰腺组织病理学评分及门静脉血内毒素含量,测定各组反应器官功能的生化指标。结果胰腺炎模型组和GW9662处理组大鼠在胰腺组织病理学评分、门静脉内毒素含量方面及各器官功能生化指标均比对照组及罗格列酮处理组明显升高(P<0.05)。结论PPARγ的配体罗格列酮能减轻实验性胰腺炎大鼠器官功能损伤,对实验性胰腺炎大鼠多器官功能有保护作用。

Objective To observe the effects of peroxisome prolifemtors-activated receptor-γ（PPARγ） on the function of the vital organs in rats with pancreatitis. Methods Acute pancreatitis （AP） was induced in 30 male SD rats by ductal injection of 4% sodium taurocholate at 1.0 ml/kg. The rats received subsequent intravenously injection of 0.3 mg/kg of PPARγ ligand （rosiglitazone, n=10）, PPARγ antagonist （GW9662, n=10） followed 10 rain later by rosiglitazone administration at 0.3 mg/kg, or left untreated （AP model group, n=10）. Another 10 male SD rats receiving no particular treatment served as the control group. The rats were sacrificed 6 h after the operation, and blood samples were collected for measurement of the biochemical indices of the vital organs. The histological changes of the pancreas and portal vein blood endotoxin content were examined. Results The rats in AP group and GW9662 group showed significantly higher level of the biochemical indices for the vital organs, pathological scores of the pancreas and portal vein blood endotoxin content were significantly higher in the control group and roglitazone-treated groups （P〈0.05）. Conclusions PPARγ ligand roglitazone can significantly ameliorate multiple organ injuries and effectively protect the functions of the organs in rats with experimental pancreatitis.