摘要
目的建立柴胡药材中总皂苷的含量测定方法。方法采用紫外可见分光光度法，以柴胡皂苷d为对照品，在波长536nm处对样品中的总皂苷进行含量测定。结果柴胡总皂苷在194～1940mg／L有良好的线性关系（r=0．9996），该法的平均加样回收率为97．35％，RSD为1．02％（n=9）。结论所建立的方法准确、简便、快速，重现性好，适用于柴胡中总皂苷的含量测定。
Objective To set up a simple and accurate method for the determination of saikosaponins in Bupleurum chinense DC. by UV - Visible Spectrophotometry. Methods The content of saikosaponins was determined by UV - Visible Spectrophotometry. The absorbency was measured at 536nm. Results The calibration curves was linear within 194 - 1940mg/L（r = 0. 9996）. The average recovery was 97.35% ,and the RSD was 1.02% （n = 9）. Conclusion The method was proved to be simple, precise and reproduciable. It can be used for the quantitative determination of B upleurum chinense DC.
出处
《医学研究杂志》
2008年第6期98-100,共3页
Journal of Medical Research
关键词
紫外可见分光光度法
柴胡
总皂苷
含量测定
UV - Visible spectrophotometry
Bupleurum chinense DC.
Saikosaponins
Content determination
