高频电针刺激对实验性肥胖大鼠脂代谢的调节作用

Effect of High-frequency Electroacupuncture on Lipid Metabolism in Obesity Rats

目的:通过对高频电针刺激肥胖大鼠后相关生化指标变化的观察,分析针刺对脂代谢的影响。方法:SD大鼠51只,随机分为对照组(n=13)、模型组(n=19)、针刺组(n=19)。采用高脂饲料、谷氨酸钠造模法建立肥胖大鼠模型。电针"后三里""三阴交""关元""中脘"穴,频率100Hz,电流3～5mA,每日1次,每次10min,共治疗28d。测定各组大鼠体重指数、左肾周脂肪重量和脂肪细胞分布;用酶学终点法测血清甘油三酯、胆固醇含量;以磷钨酸-镁沉淀法测血清高密度脂蛋白含量,聚乙烯硫酸沉淀法测血清低密度脂蛋白含量;免疫比浊终点法测定血浆脂蛋白脂酶活性;用放射免疫法测血清胰岛素、瘦素含量。结果:与模型组比较,针刺组体重指数、左肾周脂肪组织湿重及脂肪细胞面积均明显降低(P<0.01),而脂肪细胞个数增加(P<0.01);血清脂质各项含量均有不同程度改善(P<0.01,0.05),血浆脂蛋白脂酶活性升高(P<0.01);血清瘦素水平及血清胰岛素水平均降低(P<0.01,0.05)。结论:高频电针刺激对肥胖大鼠脂代谢具有良性调节作用。

Objective To observe the effect of high-frequency electroacupuncture （EA） on lipid metabolism in obesity rats. Methods Fifty-one SD rats were randomized into control group （ n = 13）, model group （ n = 19） and EA group （n = 19）. Obesity model was duplicated by feeding the rats with high fat forage and subcutaneous injection of 15 % glutamate sodium solu- tion. EA （100 Hz, 3-5 mA）was applied to ＂Housanli＂ （ST 36）, ＂Sanyinjiao＂ （SP 6）, ＂Guanyuan＂ （CV 4） and ＂Zhongwan＂ （CV 12） for 10 min everyday and for 28 days. Lee＇s index, body weight, area and the number of the adipose cells around the left kidney were detected. Blood samples were collected for assaying serum triglyceride （TG）, total cholesterol （TO） with cholesterol oxidase phenol 4-aminoantipyrine peroxidase （CHOD-PAP） method, glycerol phosphate oxidase-p-aminophenazone （GPO-PAP） methods, high density lipid-cholesterol （HDL-C） with phosphotungstic acid- Mg2＋ method, low density lipid-cholesterol （LDL-C） with polyvingel sulfate （PVS） sedimentation method and plasma lipoprotein lipase （LPL） with immunoturbidmetry endpoint method, and serum insulin and leptin contents with radioimmunoassay respectively. Results After modeling, in comparison with control group, Lee＇s index, serum TG, TO, LDL-C, fat weight, adipose cell area, serum leptin and insulin contents in model group increased considerably （P〈0.05, 0.01 ）, while serum HDL-C, plasma LPL, adipose cell number decreased significantly （P〈0.05, 0.01）. Following EA, compared with model group, Lee＇s index, serum TG, TC, LDL-C, fat weight, adipose cell area, serum leptin and insulin levels decreased significantly in EA group （P〈0.05, 0.01 ）, whereas serum HDL-C, plasma LPL, and adipose cell number increased apparently （P〈0.01）. Conclusion High frequency EA can effectively improve abnormal lipid metabolism, and reduce fat accumulation in obesity rats, which may be closely associated with its effect in regulating the balance between leptin and insulin levels.