CO_2人工气腹对人子宫内膜癌裸鼠移植瘤β_1-整合素与E-钙黏素表达的影响

Effect of CO_2 pneumoperitoneum on expressions of β_1-integrin and E-cadherins in endometrial cancer cells transplanted into nude mouse

目的探讨CO2人工气腹对子宫内膜癌裸鼠移植瘤β1-整合素与E-钙黏素表达的影响。方法取30只裸鼠分为3组:开腹组(10只),进腹后将小肠外置腹腔5min放回腹腔并缝合,右下腹腔注射肿瘤细胞。40min后排除气体。40min气腹组(10只),经腹腔内灌注CO2气体形成4mmHg的腹腔压力建立人工气腹5min后,右下腹腔注射肿瘤细胞。40min后排出气体。80min气腹组(10只),形成气腹80min后排出气体。记录每只裸鼠成瘤时间,4周后,取出移植瘤,一部分进行石蜡切片,HE染色观察移植瘤组织形态,一部分进行冰冻切片,用免疫荧光法检测各组肿瘤标本中的β1-整合素与E-钙黏素的表达,进行定量分析。结果2个气腹组成瘤时间均比开腹组短(P<0.05),HE染色中气腹组肿瘤组织中血管多。β1-整合素在子宫内膜癌裸鼠移植瘤组织的表达,2个气腹组的平均光密度(OD)值均显著小于开腹组(P<0.01),80min组表达低于40min组(P<0.05)。E-钙黏素在子宫内膜癌裸鼠移植瘤组织的表达与β1-整合素的相似,开腹组的OD值高于气腹组(P<0.01),40min组与80min组之间无显著差异(P>0.05)。结论CO2气腹环境能影响肿瘤细胞表面黏附分子β1-整合素和E-钙黏素等的表达,进而增加子宫内膜癌细胞的转移黏附能力。

Objective To discuss the effect of CO2 artificial pneumoperitoneum on the invasion of the endometrial cancer in nude mouse resulted from the transplantation of the cancer ceils and its mechanism. Methods Thirty nude mice were divided into 3 groups based on the time in the CO2 pneumoperitoneum circumstance. Control group ： the small intestine of the nude mouse was exposed in air for 5 min, and the cancer ceils were injected into right lower quadrant after suture. The gas was depleted after 40 min. 40 min group ： CO2 gas was poured into abdominal cavity to form a 4 mmHg artificial pneumoperitoneum for 5 min before cancer ceils were injected into right lower quadrant. The gas were depleted after 40 min. 80 min group ： CO2 gas was poured into abdominal cavity to form a 4 mmHg artificial pneumoperitoneum for 5 min before cancer cells were injected into right lower quadrant. The gas were depleted after 80 min. The time that each group took to form a solid tumor was recorded. Four weeks later, the transplantation tumors were taken out and sliced into frozen sections and paraffin -embedded sections. The expression of β1-intergrin and E-cadherins was detected by IMF. Results The time taken to form the solid cancer was shorter in the 40 min group and 80 min group than in the control group, with more blood vessel found （ P 〈 0. 05 ）. β1-intergrin expression in the 2 test groups was obviously lower that that in the control group （ P 〈0. 01 ） and the 80 min group was lower than the 40 min group （ P 〈 0. 05 ）. E-cadherins expression in the control group was higher than that in the test group. （ P 〈 0. 01 ） , and no difference was found in the 2 test groups （ P 〉 0. 05）. Conclusion The CO2 pneumoperitoneum could enhance the abilities of invasion and adhesion of endometrial cancer cells, which is associated with the expression changes of β1-intergrin and E-cadherins in the cancer cells.