ELISA测定血清卵巢癌抗原CA125

Enzyme linked immunosorbent assay for the quantitative measurement of ovarian caner antigen CA125 in human serum

为了能早期诊断卵巢癌，改善其预后，提高其生存率。本文应用ＥＬＩＳＡ测定了２５６例血清中ＣＡ１２５抗原，其中包括卵巢癌２７例，卵巢癌术后随访１２例，其它癌１２例，妇科良性肿瘤７８例，其它妇科疾病２３例及正常对照组１０４例。结果显示，２７例卵巢癌其ＣＡ１２５抗原检出率为９３％（２５／２７），２５６例血清样品灵敏度测定为８２％，特异性为７９％，总诊断有效率达８０％。结果提示ＣＡ１２５抗原ＥＬＩＳＡ法测定可能对卵巢癌的早期诊断及疗效观察有一定临床意义。

The objective of the study was able to detect early stage ovarian cancer and improve the survival rate of it. An enzyme linked immunosorbent assay(ELISA)was developed to measure CA125 antigen concentration in serum. A panel of 256 blinded serum samples were examined from six groups of people:27 ovarian cancer, 12 post operation of ovarian cancer, 12 other's cancer, 78 benign fumor, 23 other's diseases and 104 normal individuals. The results indicate that CA125 positive rate of ovarian was 93%(25/27), The assay of 256 samples had a sensitivity of 82% and a specificity of 79%, for an overall accuracy of 80%. The paper pointed out that CA125 assay may be significantly for diagnosing early stage ovarian cancer and observing the curative effect of patients.