液体充气鲁米诺化学发光测定全血一氧化氮

Luminol chemiluminescence detection of blood nitric oxide in headspace gas

目的建立一种新型化学发光测定一氧化氮方法。方法用氩气将全血样品中一氧化氮（ＮＯ）排出进入发光仪中鲁米诺－过氧化氢（Ｈ２Ｏ２）体系，ＮＯ与Ｈ２Ｏ２反应氧化鲁米诺产生发光。用碘化钾（ＫＩ）在酸性环境中使部份ＮＯ代谢产物还原为ＮＯ，增加方法的灵敏度。用ＥＤＴＡ为络合剂，无水乙醇为去泡剂，去除金属离子和血气泡的干扰。结果ＮＯ含量与发光强度呈正相关，１２０～１０００ｐｍｏｌＮＯ含量范围内有良好的线性关系（ｒ＝０．９９８）。全血一氧化氮浓度为６．５５μｍｏｌ／Ｌ时，批内变异系数（ＣＶ）为８．７１％。结论该方法用于全血ＮＯ测定，灵敏、特异，可避免液体介质中各种因素干扰。

Objective To establish a method of chemiluminescence for measuring blood nitric oxide (NO). Methods Nitric oxide in blood was driven out and introduced to chemiluminescence detector with a stream of argon. NO reacted with H 2O 2 to form peroxynitrite (ONOO -). Which was a stronger oxidant and could oxidize luminol to generate chemiluminescence. Results There was a linear relationship between the amount of NO and the chemiluminescence intensity over the range of 120 pmol～1 000 pmol of NO. Conclusions The assay was described in this paper was rapid, sensitive and specific and the interfere factors in liquid could be avoided.