摘要
目的建立一种测定乳酸的新方法。方法采用乳酸氧化酶氧化乳酸产生丙酮酸和Ｈ２Ｏ２，然后应用Ｔｒｉｎｄｅｒ显色反应测定血浆中乳酸。结果该方法乳酸氧化酶用量为２００Ｕ／Ｌ，酶反应时间１０分钟，线性范围可达１１ｍｍｏｌ／Ｌ，变异系数（ＣＶ）为２．３１％～３．２５％，回收率为９８．４％～１０１．０％，与法国生物－梅里埃试剂盒比较具有良好的相关，ｒ＝０．９８５，Ｙ＝０．９８７Ｘ＋０．０４０，４０例健康人血浆乳酸值为０．６０～２．５２ｍｍｏｌ／Ｌ（ｘ±２ｓ）。结论乳酸测定用乳酸氧化酶法测定乳酸具有快速、简便、灵敏、可靠等优点，适合临床应用。
Objective To develop a method of lactate oxdase for measuring lactate in plasma. Methods Lactate was oxidized by lactate oxidase to pyruvate and hyarogen peroxide, which in presence of peroxidase reactsed with trinder forming a compound. The colour in tensity of which was propotional to the concertration of lactate in the examined sample. Results The assay contained 200 U/L of lastate oxidase, and enzyme reaction time was 10min. The linearlity of tast was up to 11mmol/L. The withinrun coefficients of variation for controls at the concentrations were 2.31% to 3.25%. The recoveries ranged from 98.4% to 101.0%. Comparing this method with biomerieux reagents method, we obtained the regression equation, r =0.985, Y =0.987 X +0.024. The reference inteval ( ±2 s ) was 0.60 to 2.52 mmol/L n =40. Conclusions This method is simple, sensitive, and suitable for clinical application.
