脾切除对肺内细菌清除和移位的影响

The effects of splenectomy on the clearance and translocation of bacterium within the lungs of rats

目的:探讨脾切除对肺内细菌清除及移位的影响,同时观察自体脾组织移植的应用效果.方法:将Wistar大鼠90只随机分为假手术组、脾切除组和半脾移植组,采用肺炎球菌悬液雾化吸入方法攻击动物,观察肺组织学病变,肺内细菌清除和移位状况.结果:脾切除组动物肺组织严重充血肿胀,炎性细胞浸润少,肺内细菌清除功能降低,细菌向肺门淋巴结移位和侵入血流加快,与假手术组比较有显著性差异(P<0.05),但半脾移植组动物能基本恢复对肺炎球菌的抵抗能力.结论:脾切除后动物肺抗菌功能降低,而自体脾组织移植是保留脾功能的有益术式.

Ninetv Wistar rats randomlv divided in- to shamoperation group, splenectomy group and splenic tissue autotransplantation group were attacked by exposure to nebulized suspension of Streptococcus Pneu-mococcl (S.Pn) for the observation of pulmonary histopathologic changes, the clearance and transloca-tion of S. Pn from lungs in order to fathom the effects of splenectomy on the clearance and translocation of bacterium within lungs and to value the applying effect of splenic tissue autotransplantation of bacterium within lungs. The lungs of splenectomized rats were severely hyperemia and swelling and their infiltration of poly-morphonuclears. the clearance of S. Pn were impaired with the enhancement of the translocation to tracheo-bronchia lymphnodes and the invasion to blood-stream of S. Pn. The difference between shamoperation group and splenectomy group was statistically significant. However, the resistance to S. Pn restored in splenic tissue autotransplantation. It was suggested that the pulmonary antibacterial function in splenectomized rats was impaired and the splenic tissue autotransplantation was a useful operation for restoring splenic function after splenectomy.