铁离子致脑出血后血肿周围组织损伤研究

Study on injury in brain parenchyma surrounding intracebral hemorrhage induced by iron ions

目的:研究脑出血后血红蛋白的代谢产物铁离子在血肿周围组织损伤中的作用及去铁胺的干预影响。方法:将SD大鼠随机分为假手术组、脑出血组和去铁胺干预组,以自体血大鼠尾状核注射为模型,观察各组大鼠建模术后不同时间点神经功能评分、脑组织含水量和血脑屏障通透性变化、血清S100B蛋白和神经元特异性烯醇化酶(Neuron-specific-enolas,NSE)水平及去铁胺干预的影响。结果:与对照组比较,脑出血模型大鼠在建模术后1～7d存在明显神经功能障碍,血肿周围脑组织水份含量明显增加,血脑屏障通透性明显增高,血清S100B蛋白和NSE水平明显升高。去铁胺干预组模型大鼠各观察指标与脑出血模型大鼠呈现相同变化趋势,但神经功能评分明显低于脑出血组,血肿周围脑组织水份含量明显低于脑出血组,血脑屏障通透性明显低于脑出血组,血清S100B蛋白和NSE水平明显低于脑出血组。结论:脑出血后铁离子异常积聚参与了脑出血后血肿周围组织的损伤,去铁胺对这种损伤有保护作用。

Objective：To investigate the role of iron ions released by metabolism products of haemoglobin after intracerebral hemorrhage（ICH） in the injury of surrounding brain parenchyma and the influence of intervention by defemxamine（DFO）. Methods：Sprague-Dawley rats were randomly divided into the sham operation groups.intracebral hemorrhage（ ICH ） groups and deferoxamine groups,and received infusion of autologous blood. Evaluation on neurological function,brain water content,the permability of blood brain barrier,SlOOB pmteins,neumn-specific-enolas（NSE） and the influence of intervention by deferoxamine were detected at different time points after operation. Results：Compared with the sham operation groups,the rats of ICH groups had significantly neurological disorder and the increase of brain water content surrounding brain parenchyma,the permeability of blood brain barrier,S100B protein and NSE of blood serum. The rats of DFO groups had the same trend with the rats of ICH groups in all kinds of observed items but neurological function scores test,brain water content surrounding brain parenchyma,the permeability blood brain barrier,SlOOB protein and NSE of blood serum were lower than those of ICH groups. Conclusion：The abnormal accumulation of iron ions participates the injury of brain tissue surrounding brain parenchyma after ICH and deferoxamine has the protective role for the injury.