高效液相色谱法同时测定蔬菜水果中5种黄酮类化合物

Simultaneous Determination of Five Flavonoid Compounds in Vegetables and Fruits by High Performance Liquid Chromatography

目的建立同时测定蔬菜水果中芹菜素、山奈酚、槲皮素、异鼠李黄素和毛地黄黄酮5种黄酮类物质的反相高效液相色谱方法。方法选用C18色谱柱（SYNERGI POLRR-RP80A，250mm×4．6mm），以甲醇-0．1％磷酸水溶液（v／v，60／40）作为流动相进行洗脱。柱温30℃，流速1．0ml／min，在339nm波长下进行检测。结果槲皮素、毛地黄黄酮、山奈酚、芹菜素和异鼠李黄素的线性范围分别为0．07～19．8、0．07～20．0、0．10～20．1、0．08～20．3和0．09～20．1μg/ml；对于全部待测组分相关系数（r）均大于0．99；检出限为0．02～0．04μg／ml。方法的实际样品加标回收率为86．4％～104．0％；相对标准偏差（兄如）低于7．6％（n=3）。结论该方法简便、快速、准确，可用于蔬菜和水果中黄酮类物质含量的测定。

Objective To establish a simple, rapid and sensitive reversed-phase high-performance liquid chromatographic method for the simultaneous determination of five flavonoid compounds in vegetables and fruits. Methods The separation was performed on a C18 column（ SYNERGI POLRR-RP80A, 250mm × 4. 6mm） with 0. 1% （v/v） phosphoric-methanol （v/v, 2/3） at 30℃, and the detection wavelength was set at 339nm with a diode array detector. Results The calibrationgraph was linear in the range of 0. 07 - 19.8, 0. 07 - 20. 0, 0. 10 - 20. 1,0. 08 - 20. 3, 0. 09 - 20. 1 μg/ml with r 〉 0. 99 for quercetin, luteolin, kaempferol, apigenin, isorhamnetin, respectively. Limits of detection varied from 0. 02 to 0. 04 μg/ml. For sample analysis, the RSD was less than 7.6% and the recoveries were from 86. 4% to 104. 0%. Conclusions The method is simple, fast, accurate and convenient, and can be applied to determine flavonoid compounds in vegetable and fruit samples.