猪圆环病毒2型感染昆明小鼠模型的建立

Establishment of a Kunming mouse model for infection with PCV2

将SPF级昆明小鼠随机分为3组,即一次攻毒组(S组)12只、连续攻毒组(M组)3只和对照组(C组)12只。S组和C组小鼠于第1d分别接种RPMI1640培养液和猪圆环病毒2型(PCV2)细胞培养毒,M组小鼠于第1、7、14、28、35d连续进行PCV2接种。S组和C组小鼠分别在接种后第7、14、28和42d各处死3只,M组小鼠在接种后第42d全部处死,分析其增重、病毒组织分布、抗体水平、病理组织学变化。结果,C组小鼠体重增长速率大于S组和M组,S组又明显高于M组;S组和M组小鼠体内存在病毒复制,而C组小鼠体内无病毒复制;S组和M组小鼠均产生PCV2抗体,但M组小鼠抗体水平明显高于S组,C组小鼠未检测到PCV2抗体;S组和M组小鼠有明显的病理损伤,其中淋巴结损伤最严重,C组未发现病理损伤。结果表明PCV2可以成功感染SPF昆明小鼠。

Twelve SPF-Kunming mice in Group S were inoculated with RPMI 1640,12 SPF-Kunming mice in the control were inoculated with a single dose of porcine circovirus type 2（PCV2） and 3 SPF-Kunming mice in Group M were injected with multiple doses of PCV2. Three mice in the control and 3 mice in Group S were sacrificed on day 7,14,28, and 42 post-inoculation（PI）, respectively. All mice in Group M were sacrificed on day 42 PI. Their body weights and levels of antibody against PCV2 were examined. The increment of body weight in the control was significantly higher than those in Group S and M,and the increment of body weight in Group S was significantly higher than that in Group M. The viral replication was detected in Group S and M, but not detected in the control. The specific antibody against PCV2 were observed in Group S and M. The level of antibody in Group M was significantly higher than that in Group S. No anti-PCV2 antibody was detected in the control. The conspicuous pathological injuries were observed in Group S and M. The lymph nodes of the mice in Group S and M were damaged seriously by PCV2. The same injury was not found in the control. The results showed that mice could be infected successfully by PCV2.