摘要
根据GenBank中登录的猪生殖与呼吸综合征病毒(PRRSV)N蛋白基因、猪圆环病毒2型(PCV-2)rep蛋白基因和猪伪狂犬病病毒(PRV)gE基因的核苷酸序列分别设计了3对特异性引物,成功建立了同时检测PRRSV、PCV-2、PRV的多重SYBR Green-Ⅰ实时荧光PCR方法。敏感性试验结果显示,PRRSV、PCV-2的敏感性可达250拷贝/μL,PRV的敏感性可达500拷贝/μL。表明,该方法具有较好的特异性、重复性和敏感性,可以用于PRRSV、PCV-2和PRV的快速检测。
Three pairs of primers were designed respectively based on the sequences of N protein gene of PRRSV, rep protein gene of PCV-2 and gE gene of PRV available in GenBank. A multiplex SYBR Green-Ⅰ real time PCR assay was established successfully for simultaneous detection of the 3 viruses. The sensitivities for PRRSV,PCV-2 and PRV were 250,250 and 500 copies/μL, respectively. The results revealed that the established PCR assay was sensitive, specific and reproducible and it could be used to detect rapidly PRRSV,PCV-2 and PRV.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2008年第6期494-499,共6页
Chinese Veterinary Science
基金
黑龙江省科技攻关计划重点项目(GB05B501-1)