摘要
为研究猪白细胞介素-6(IL-6)作为免疫佐剂的可行性,用IPTG诱导含有猪IL-6基因的BL21(DE3)LysS工程菌,表达的目的蛋白经超声波破碎,洗涤,8mol/L尿素变性溶解后,用固化金属亲和层析法纯化后用谷胱甘肽再氧化还原法进行复性,并用MTT法对复性的重组蛋白的生物学活性进行分析。结果显示,复性的重组猪IL-6具有良好的刺激淋巴细胞体外增殖的活性。说明重组猪IL-6在作为免疫佐剂方面有一定的应用价值。
To explore the feasibility of porcine interleukin-6 protein（plL-6） as immunoadjuvant,Escherichia coli BL21（DE3） LysS with the gene encoding plL-6 was induced by IPTG. The expressed inclusion bodies were solubilized and denatured in the presence of 8 mol/L urea,purified by Immoble Metal Affinity Chromatography（IMAC） and renatured with GSH/GSSH. The lymphocyte proliferation activity of the renatured protein pIL-6 was analyzed by using MTT colorimetric analysis. The MTT result showed that the renatured pIL-6 had good stimulating activity for lymphocyte proliferation,indicating that the pIL-6 may have potential as immunoadjuvant.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2008年第6期531-534,共4页
Chinese Veterinary Science
基金
国家“十五”科技攻关计划项目重大专项(2001AA213141)
关键词
猪
IL-6基因
表达
纯化
复性
淋巴细胞增殖活性
porcine
IL-6 gene
expression
purification
renaturation
lymphocyte proliferation activity
