HBV-DNA阳性育龄妇女HBV血清标志物、前S1抗原的检测分析

Study on the Determination of Serum HBV Markers and Pre- S1 Antigen in Child - bearing Age Women with Positive HBV - DNA （over 10^3 copies/ml）

目的:探讨HBV-DNA阳性育龄妇女病毒复制与HBV标志物及前S1抗原的关系。方法:对1643例慢性乙型肝炎育龄妇女采用荧光定量PCR法检测血清HBV-DNA,酶联免疫吸附法(ELISA)检测HBV标志物和前S1抗原。结果:HBV-DNA阳性育龄妇女432例。其中,HBsAg阴性者占19.44%,前S1抗原总阳性率55.09%,且随着HBV-DNA载量增加,前S1抗原阳性率也升高。结论:采用HBV-DNA、HBV标志物、前S1抗原联检,才能更准确提供育龄妇女HBV感染诊疗依据,有效控制HBV感染率。

Objoctive To study the positivity of serum HBV markers （ HBsAg, HBsAb, HBeAg, HBeAb, HBcAb） and Pre - S1 antigen in child - beating age women with serum HBV - DNA concentrations over 10^3 copies/ml. Methods Serum HBV - DNA con-tents were determined with FQ - PCR in 1643 child - bearing age women with documented history of chronic HBV hepatitis. Serum HBV-markers （HBsAg, HBsAb, HBeAg, HBeAb, HBcAb） and pre - S1 antigen wree detected with ELISA in 432 of the 1643 women with HBV - DNA contents over 10^3 copies/ml, Results Serum HBsAg was negative in 19.44% （ n = 84） of the 432 women with positive HBV - DAN. Serum pre - S1 antigen was negative in 44, 91% （ n = 194） and positive in 55. 09% （ n = 238） of the 432 women. Positivlty pre - S1 antigen increased along with the increase of HBV - DNA contents. Conclusion For detection of HBV infection, determinations of HBV -DNA, HBV markers and pre -S1 antigen were mutually complementary and combined determinations would be most desirable.