骨肿瘤DNA流式细胞术定量分析

Quantitative Analysis of DNA in Bone Tumors by Flow Cytometry

应用流式细胞术对６２例未经化疗或放疗的原发骨肿瘤的石蜡包埋标本细胞核ＤＮＡ进行了测定，其中３例为良性肿瘤或瘤样病变，３３例为骨巨细胞瘤，２６例为其他恶性骨肿瘤。结果表明，良性骨肿瘤与瘤样病变中未见异倍体，骨巨细胞瘤中ＤＮＡ异倍体的发生率也很低，而其他恶性骨肿瘤中异倍体的发生率非常之高，故异倍体可作为恶性骨肿瘤的一个较特异的标志。且单个骨肿瘤在活检与大体标本、原发与复发肿瘤上表现了稳定的倍性，而基于病理学基础上的骨肉瘤的分型与分级在ＤＮＡ含量上并无明显的区别。

The nuclear DNA content in 62 primary bone tumors without administration of chemotherapy or radiotherapy before operation was measured by flow cytometry(FCM) on paraffin embedded specimens. 3 were benign or tumorlike lesions, 33 were giant cell tumor of bone(GCT), 26 were malignant. There was no aneuploidy in benign or tumorlike lesions, a low percentage in GCT, but much more higher proportion of aneuploidy was found in malignant tumors. Thus DNA aneuploidy was a specific marker of malignant bone tumors. The results also showed that, the biopsy and the surgical specimens had the same DNA ploidy, so did the primary and the recurrent lesions, but no difference of DNA content and ploidy was displayed between the distinct types and grades of osteosarcomas.