载脂蛋白B基因多态性与I型糖尿病并冠心病关系的研究

A STUDY ON THE RELATIONSHIP BETWEEN APOB GENE POLYMORPHISM AND CHD IN NIDDM

应用聚合酶链反应（ＰＣＲ）技术对非胰岛素依赖型糖尿病（ＮＩＤＤＭ）４１例、ＮＩＤＤＭ合并冠心病５１例和正常对照组６０例进行载脂蛋白Ｂ基因ＥｃｏＲＩ和ＸｂａＩ酶切位点限制性片段长度多态性（ＲＦＬＰ）研究。结果显示少见等位基因频率在ＮＩＤＤＭ组及ＮＩＤＤＭ并冠心病组均升高。与对照组比较，Ｅ－等位基因频率在糖尿病患者显著升高。

The relationship between CHD in NIDDM and apolipoprotein B(apoB) gene polymorphism was studied using polymerase chain reaction and two polymorphic sites of apoB (EcoRI and XbaI) were examined in samples from 41 patients with NIDDM, 51 patients of NIDDM with CHD and 60 healthy individuals as control. The results were as follows: 1. The frequencies of the rarer alleles of the EcoRI and XbaI (E-,X+) were higher in NIDDM with or without CHD. 2. The frequencies of E- alleles were over_represented in NIDDM. 3. The frequencies of X+ alleles were markedly correlated with CHD in NIDDM.