从形态和超微结构对干燥保存角膜内皮细胞活性的评价

The endothelial cellular activities of corneas preserved by desiccation evaluated on the morphology and ultrastructure

为从形态和超微结构上观察干燥保存角膜内皮细胞是否具有活性，应用０．２％茜素红和０．２５％台盼兰对干燥保存不同时间的兔、猴和人角膜内皮细胞分别进行活性染色，并与正常新鲜者作对照。同时，在电镜下对干燥保存角膜及其新鲜标本进行超微结构观察。结果，新鲜角膜六角形细胞边界清楚，核呈淡蓝色；干燥保存角膜，六角形细胞边界不清，但核的染色及分布密度与新鲜者几无区别。电镜下，干燥保存角膜六角形镶嵌状细胞边界看不到，细胞间连接处有空泡，但细胞轮廓可见；核染色质有皱缩，但无核溶解现象。胞浆内有空泡，但仍有细胞器存在；人的细胞膜上还可看到微绒毛。结论：干燥保存角膜无活性特征虽然明显，但也存在有活性的标志。

Objective It is observed on the morphology and ultrastructure whether the endothelial cells of the corneas preserved by desiccation have activities.Method The endothelial cells of the corneas preserved by desiccation of rabbits rhesus monkey and Human for various duration were actively stained by 0 2% alizarin red S and 0 25% trypan blue respecticely,the endothelial cells of norrmal fresh corneas as control.At one time,ultrastructures of the corneas preserved by desiccation were observed under electron microscope.Results The endothelial hexagonal cellular border of normal fresh corneas were clear and nucleus appeared light blue,but the endothelial cellular borders of corneas preserved by desiccation were no clear,however staining and density of the endothelial cellular nuclei between fresh and corneas preserved by desiccation all but were no difference.Under electron microscopy,the endothelial cells arranged in mosaics like with hexagonal borders of the corneas preserved by desiccation could no be seen,there were vacuoles between the cells,but could be cellular outline.Nucleus chromatin had pyknosis but no karyolysis.Vacuoles in cytoplasm could be seen but also organellae and microvilli on the cellular membrane of Human.Conclusion Although the endothelial cells of the corneas preserved by desiccation have clearly no vital signs yet have vital signs.