射干药材HPLC指纹图谱研究

HPLC Fingerprint of Rhizoma Belamcandae

目的:建立射干药材的HPLC指纹图谱。方法:采用反相高效液相色谱(RP-HPLC)法建立指纹图谱,色谱柱为Lichrospher C18(250mm×4.6mm,5μm),流动相为1%醋酸乙腈-1%醋酸水溶液,流速为1mL.min-1,柱温为30℃;以峰面积为量化特征值进行聚类分析,用逐步分类法建立射干指纹图谱的典则判别函数和Fisher's判别函数。结果:构建了射干药材的指纹图谱;两类函数对样品判别分类结果与原分类完全相同,判别相符率为100%。结论:两类判别函数的建立,对未知样品的鉴别提供了一个准确、快捷的工具,为中药的质量控制提供了一种新的模式,具有理论意义和实用价值。

OBJECTIVE： To establish the PIPLC fingerprint of Rhizoma Belamcandae. METHODS： The fingerprint of Rhizoma Belamcandae was established by HPLC on Lichrospher C18（ 250 mm× 4.6mm, 5um）. The mobile phase consisted of 1% acetic acid acetonitrile - 1% acetic acid solution at a flow rate of 1.0 mL · min^-1. The column temperature was 30 ℃ .Cluster analysis was conducted with peak area as quantitative characteristic value. Canonical discriminant function and Fisher＇ s discriminant function of the HPLC finger print of Rhizoma Belamcanda were established using stepwise classification scheme. RESULTS： The fingerprint of Rhizoma Belamcandae has been established. The discrimination and classification results by the two kinds of functions were totally consistent with each other, with corresponding rate of 100%. CONCLUSION： The two discriminant functions are of theoretical and practical value for they provide an accurate and rapid tool for the identification of the unknown samples as well as a new model for the quality control of Chinese medicines.